Table 2 Oligonucleotides used in this study
From: Corynebacterium glutamicum as platform for the production of hydroxybenzoic acids
Oligonucleotide | Sequence (5′–3′) | Restriction site |
|---|---|---|
del_cg3349-54-up-s | ACCAAGCTTCATTTTCTAGTTGAGATTCATTATCATAACGCTTGACAGTACAACTATGTC | HindIII |
del_cg3349-54-up-as | GCTTCCGGGGTGAATGACGGAGCCGCTGCGGAGGTTCCAGAGCCACCGCGCTG | – |
del_cg3349-54-down-s | CGCAGCGGCTCCGTCATTCACCCCGGAAGCTGGCGCTACGCCCTCCTCGAAC | – |
del_cg3349-54-down-as | ACCTCTAGATTTCTGTCTTGAGGGTTCGTGGGGGCTG | XbaI |
check-cg3349-54-s | AAAGGCTCCATGAATTCCTCACGGAGGATCTC | – |
check-cg3349-54-as | ACATCACAAGTAGAAACCCGCATTTTCTGTAGTTTTTAC | – |
(A)-aroF*-s | TTCGCTCTTCAAAGCTGCTTAAGGAGGCTATCTATGACCGATGAACAGGTGCTGATGACCCCAG | SapI |
(A)-aroF*-as | TACGCTCTTCTGATTTATGCCACGCGTGCGGTCAGCTG | SapI |
(A)-aroH-s | TTCGCTCTTCAAAGTATACCATGGTAAGGAGGTTCAGCATGAACAGAACTGACGAACTCCGTACTGCGCGTATTG | SapI |
(A)-aroH-as | TACGCTCTTCTGATTTAGAAGCGGGTATCTACCGCAGAGGCGAGTTTTTC | SapI |
(B)-qsuB-s | TTCGCTCTTCAATCTCTATCAAGGAGGATCGGCATGCGTACATCCATTGCCACTGTTTGTTTGTC | SapI |
(B)-qsuB-as | TACGCTCTTCTTCGTTAGTTTGGGATTCCCCGCTCGAGGTC | SapI |
(B)-irp9-s | TTCGCTCTTCAATCTCTATCAAGGAGGATCGGCATGAAGATCTCCGAGTTCCTCCACCTGGCAC | SapI |
(B)-irp9-as | TACGCTCTTCTTCGTTACACCATCAGGTATGGTGCAATGGAGGCCAGCTTTTCGCGAGTTTCG | SapI |
(B)-hyg5-s | TTCGCTCTTCAATCTCTATCAAGGAGGATCGGCATGAACCCATCCTCCTTGGTGCTGAAC | SapI |
(B)-hyg5-as | TACGCTCTTCTTCGTTACATGACCACGCCTTCGATTTCCACGAG | SapI |
(B)-ubiC-s | TTCGCTCTTCAATCTCTATCAAGGAGGATCGGCATGTCACACCCCGCGTTAACGCAACTG | SapI |
(B)-ubiC-as | TACGCTCTTCTTCGTTAGTACAACGGTGACGCCGGTAAAAACAGTTCTGTTAG | SapI |
tkt’-s | CATGGATCCAAGGAGGTTCAGCATGACCACCTTGACGCTGTCACCTGAACTTCAG | BamHI |
tkt’-as | AGAGCTCTTCAGCCCATAGCGTGCTCACGGATACCGAAGTG | SapI |
‘tkt-s | TTTGCTCTTCTGGCTCCATCCTCAACGGCATTTCCCTCC | SapI |
‘tkt-as | TCTGAATTCTTAACCGTTAATGGAGTCCTTGGCCGCTGCCAC | EcoRI |
pMKEx2_own-s | CCCTCAAGACCCGTTTAGAGGC | – |
pMKEx2_own-as | TTAATACGACTCACTATAGGGGAATTGTGAGC | – |
pEKEx3-s | GCAAATATTCTGAAATGAGCTGTTGACAATTAATCATC | – |
pEKEx3-as | CGTTCTGATTTAATCTGTATCAGGCTGAAAATCTTCTC | – |