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Table 6 Metabolite analysis of culture broths of the K. pneumoniae Δ(ldhA als) mutant by repeated fed-batch fermentation at intervals of 24 h using crude glycerol as a substrate under different pH conditions

From: Efficient production of 1,3-propanediol from crude glycerol by repeated fed-batch fermentation strategy of a lactate and 2,3-butanediol deficient mutant of Klebsiella pneumoniae

Cycle no. 1,3-PDO (g/L) 2,3-BDO (g/L) Ethanol (g/L) Acetate (g/L) Succinate (g/L) 1,3-PDO productivity (g/L/h)
pH 6.0 6.5 7.0 6.0 6.5 7.0 6.0 6.5 7.0 6.0 6.5 7.0 6.0 6.5 7.0 6.0 6.5 7.0
1 49.9 53.5 45.5 10.1 11.1 9.5 4.1 7.5 8.9 6.0 5.0 4.0 3.89 6.47 7.87 2.08 2.23 1.90
2 49.4 54.2 49.8 11.2 11.3 11.6 3.9 5.7 10.4 4.9 4.6 4.4 3.29 6.04 7.34 2.06 2.26 2.08
3 49.7 57.7 44.6 10.5 12.7 10.7 4.8 7.8 10.6 6.3 5.4 3.2 3.36 6.59 7.92 2.07 2.40 1.86
4 47.4 54.4 48.1 8.1 11.8 10.4 2.4 6.2 7.5 5.7 5.0 3.0 3.65 5.99 7.39 1.98 2.27 2.00