Fig. 5From: Efficient production of 1,3-propanediol from crude glycerol by repeated fed-batch fermentation strategy of a lactate and 2,3-butanediol deficient mutant of Klebsiella pneumoniaeConfirmation of construction of the Δ(ldhA adc) (a) and Δ(ldhA ard) mutants (b) of K. pneumonia Cu by Southern hybridization. Probes: adc-up DNA sequences upstream of adc gene, ard-up DNA sequences upstream of ard gene, Apr apramycin resistance gene [aac(3)IV]Back to article page