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Table 3 Products’ accumulation during transformation in Isaria farinosa KCh KW1.1 culture

From: Biotransformation of steroids by entomopathogenic strains of Isaria farinosa

Substrate

Compounds found in the reaction mixture (%)

Biotransformation time (days)

1

3

7

Adrenosterone

ADR

9 ± 2.6

6β-OH-ADR

46 ± 2.8

52 ± 2.2

49 ± 3.6

Progesterone

P

6β,11α-OH-P

74 ± 1.3

71 ± 2.4

64 ± 3.1

6β-OH,11-oxo-P

4 ± 0.7

5 ± 1.1

3 ± 0.9

1,4,6-triene-T

1,4,6-triene-T

5 ± 1.3

15α-OH-6,7β-epoxy-1-ene-T

37 ± 2.2

35 ± 1.4

32 ± 1.8

14α-OH-6,7β-epoxy-1-ene-T

20 ± 0.3

19 ± 0.2

16 ± 1.3

15α-OH-6,7β-epoxy-T

10 ± 0.8

13 ± 2.0

17 ± 2.4

  1. Biotransformation conditions: 100 mL of cultivation medium (3% glucose, 1% bacteriological peptone) in 300 mL Erlenmeyer flasks, 24 °C, 150 r/min for 7 day
  2. Data are the average of three independent experiments. Standard errors were indicated