Table 2 Composition of the crude mixture obtained in the transformation of DHEA in Isaria farinosa strains
From: Biotransformation of steroids by entomopathogenic strains of Isaria farinosa
Isaria farinosa strain | Compounds found in the reaction mixture (%) | Biotransformation time (h) | ||||||
|---|---|---|---|---|---|---|---|---|
3 | 6 | 9 | 12 | 24 | 72 | 168 | ||
KCh J1.1 | DHEA | 100 ± 0.1 | 100 ± 0.1 | 99 ± 0.3 | 93 ± 0.5 | 74 ± 3.9 | – | – |
7α-OH-DHEA | – | – | – | 5 ± 0.7 | 17 ± 2.7 | 60 ± 3.0 | 43 ± 4.6 | |
7β-OH-DHEA | – | – | – | 2 ± 0.5 | 9 ± 2.3 | 38 ± 2.6 | 42 ± 3.8 | |
KCh J1.2 | DHEA | 100 ± 0.1 | 100 ± 0.3 | 72 ± 3.3 | 39 ± 4.1 | – | – | – |
7α-OH-DHEA | – | – | 21 ± 1.6 | 35 ± 1.8 | 64 ± 0.9 | 46 ± 1.1 | 14 ± 3.3 | |
7β-OH-DHEA | – | – | 7 ± 1.2 | 14 ± 2.0 | 36 ± 1.3 | 52 ± 1.1 | 46 ± 4.2 | |
KCh J1.3 | DHEA | 100 ± 0.1 | 96 ± 0.5 | 63 ± 3.7 | 21 ± 3.9 | 1 ± 1.0 | – | – |
7α-OH-DHEA | – | 3 ± 0.5 | 16 ± 2.5 | 49 ± 1.6 | 64 ± 0.7 | 35 ± 3.3 | – | |
7β-OH-DHEA | – | 1 ± 0,2 | 5 ± 1.2 | 21 ± 1.8 | 25 ± 0.5 | 50 ± 3.2 | – | |
KCh J1.4 | DHEA | 100 ± 0.2 | 96 ± 0.5 | 75 ± 2.1 | 44 ± 2.7 | 4 ± 0.6 | – | – |
7α-OH-DHEA | – | 3 ± 0.1 | 12 ± 1.1 | 33 ± 0.9 | 61 ± 3.2 | 51 ± 4.3 | – | |
7β-OH-DHEA | – | 1 ± 0.1 | 4 ± 0.2 | 13 ± 1.2 | 24 ± 1.9 | 36 ± 3.9 | – | |
KCh J1.6 | DHEA | 100 ± 0.1 | 87 ± 0.8 | 36 ± 3.4 | 4 ± 0.5 | 1 ± 0.3 | – | – |
7α-OH-DHEA | – | 8 ± 1.1 | 41 ± 2.9 | 66 ± 3.6 | 54 ± 1.1 | 26 ± 2.3 | – | |
7β-OH-DHEA | – | 2 ± 0.3 | 16 ± 2.0 | 24 ± 1.7 | 30 ± 2.3 | 46 ± 4.2 | – | |
KCh J2.2 | DHEA | 100 ± 0.3 | 88 ± 2.0 | 55 ± 3.1 | – | – | – | – |
7α-OH-DHEA | – | 6 ± 0.8 | 31 ± 2.7 | 70 ± 0.9 | 67 ± 2.0 | 50 ± 1.6 | – | |
7β-OH-DHEA | – | 2 ± 0.7 | 11 ± 1.1 | 26 ± 0.4 | 29 ± 2.6 | 47 ± 1.3 | – | |
KCh J2.3 | DHEA | 100 ± 0.1 | 97 ± 0.4 | 90 ± 1.6 | 79 ± 0.9 | – | – | – |
7α-OH-DHEA | – | 2 ± 0.4 | 7 ± 1.3 | 16 ± 1.7 | 62 ± 0.3 | 45 ± 1.5 | 41 ± 4.3 | |
7β-OH-DHEA | – | 1 ± 0.3 | 3 ± 0.8 | 6 ± 2.1 | 31 ± 0.7 | 47 ± 2.3 | 39 ± 2.8 | |
KCh J2.4 | DHEA | 100 ± 0.6 | 84 ± 0.9 | 48 ± 3.2 | 3 ± 0.6 | – | – | – |
7α-OH-DHEA | – | 11 ± 2.4 | 28 ± 4.1 | 65 ± 1.6 | 62 ± 1.3 | 40 ± 1.7 | 2 ± 1.0 | |
7β-OH-DHEA | – | 5 ± 0.7 | 10 ± 1.4 | 21 ± 2.0 | 32 ± 2.2 | 42 ± 2.2 | 13 ± 3.2 | |
KCh J2.6 | DHEA | 100 ± 0.3 | 100 ± 0.5 | 81 ± 4.5 | 27 ± 3.8 | 6 ± 2.0 | 3 ± 1.1 | – |
7α-OH-DHEA | – | – | 14 ± 3.1 | 38 ± 4.1 | 47 ± 1.4 | 34 ± 1.9 | – | |
7β-OH-DHEA | – | – | 5 ± 2.6 | 15 ± 3.1 | 28 ± 1.8 | 49 ± 2.1 | – | |
KCh KW1.1 | DHEA | 100 ± 0.2 | 80 ± 2.1 | 53 ± 2.8 | 1 ± 0.6 | 1 ± 0.8 | – | – |
7α-OH-DHEA | – | 12 ± 1.3 | 21 ± 2.6 | 68 ± 3.9 | 49 ± 1.3 | 34 ± 2.9 | 24 ± 2.7 | |
7β-OH-DHEA | – | 4 ± 0.7 | 8 ± 1.5 | 26 ± 0.6 | 39 ± 0.4 | 56 ± 3.1 | 56 ± 3.0 | |
KCh KW1.2 | DHEA | 100 ± 0.3 | 95 ± 1.7 | 68 ± 3.9 | – | – | – | – |
7α-OH-DHEA | – | 4 ± 0.6 | 22 ± 2.0 | 69 ± 0.7 | 50 ± 1.3 | 34 ± 2.6 | 23 ± 2.3 | |
7β-OH-DHEA | – | 1 ± 0.3 | 8 ± 1.9 | 27 ± 0.9 | 40 ± 0.5 | 56 ± 3.0 | 57 ± 4.4 | |
KCh RJ1.1 | DHEA | 100 ± 0.1 | 100 ± 0.8 | 97 ± 0.7 | 27 ± 1.6 | 26 ± 3.3 | – | – |
7α-OH-DHEA | – | – | 2 ± 0.2 | 38 ± 0.9 | 46 ± 1.7 | 51 ± 0.2 | 40 ± 3.0 | |
7β-OH-DHEA | – | – | 1 ± 0.3 | 16 ± 0.7 | 23 ± 2.8 | 46 ± 1.5 | 57 ± 2.4 | |