Skip to main content

Advertisement

Table 2 Composition of the crude mixture obtained in the transformation of DHEA in Isaria farinosa strains

From: Biotransformation of steroids by entomopathogenic strains of Isaria farinosa

Isaria farinosa strain Compounds found in the reaction mixture (%) Biotransformation time (h)
3 6 9 12 24 72 168
KCh J1.1 DHEA 100 ± 0.1 100 ± 0.1 99 ± 0.3 93 ± 0.5 74 ± 3.9
7α-OH-DHEA 5 ± 0.7 17 ± 2.7 60 ± 3.0 43 ± 4.6
7β-OH-DHEA 2 ± 0.5 9 ± 2.3 38 ± 2.6 42 ± 3.8
KCh J1.2 DHEA 100 ± 0.1 100 ± 0.3 72 ± 3.3 39 ± 4.1
7α-OH-DHEA 21 ± 1.6 35 ± 1.8 64 ± 0.9 46 ± 1.1 14 ± 3.3
7β-OH-DHEA 7 ± 1.2 14 ± 2.0 36 ± 1.3 52 ± 1.1 46 ± 4.2
KCh J1.3 DHEA 100 ± 0.1 96 ± 0.5 63 ± 3.7 21 ± 3.9 1 ± 1.0
7α-OH-DHEA 3 ± 0.5 16 ± 2.5 49 ± 1.6 64 ± 0.7 35 ± 3.3
7β-OH-DHEA 1 ± 0,2 5 ± 1.2 21 ± 1.8 25 ± 0.5 50 ± 3.2
KCh J1.4 DHEA 100 ± 0.2 96 ± 0.5 75 ± 2.1 44 ± 2.7 4 ± 0.6
7α-OH-DHEA 3 ± 0.1 12 ± 1.1 33 ± 0.9 61 ± 3.2 51 ± 4.3
7β-OH-DHEA 1 ± 0.1 4 ± 0.2 13 ± 1.2 24 ± 1.9 36 ± 3.9
KCh J1.6 DHEA 100 ± 0.1 87 ± 0.8 36 ± 3.4 4 ± 0.5 1 ± 0.3
7α-OH-DHEA 8 ± 1.1 41 ± 2.9 66 ± 3.6 54 ± 1.1 26 ± 2.3
7β-OH-DHEA 2 ± 0.3 16 ± 2.0 24 ± 1.7 30 ± 2.3 46 ± 4.2
KCh J2.2 DHEA 100 ± 0.3 88 ± 2.0 55 ± 3.1
7α-OH-DHEA 6 ± 0.8 31 ± 2.7 70 ± 0.9 67 ± 2.0 50 ± 1.6
7β-OH-DHEA 2 ± 0.7 11 ± 1.1 26 ± 0.4 29 ± 2.6 47 ± 1.3
KCh J2.3 DHEA 100 ± 0.1 97 ± 0.4 90 ± 1.6 79 ± 0.9
7α-OH-DHEA 2 ± 0.4 7 ± 1.3 16 ± 1.7 62 ± 0.3 45 ± 1.5 41 ± 4.3
7β-OH-DHEA 1 ± 0.3 3 ± 0.8 6 ± 2.1 31 ± 0.7 47 ± 2.3 39 ± 2.8
KCh J2.4 DHEA 100 ± 0.6 84 ± 0.9 48 ± 3.2 3 ± 0.6
7α-OH-DHEA 11 ± 2.4 28 ± 4.1 65 ± 1.6 62 ± 1.3 40 ± 1.7 2 ± 1.0
7β-OH-DHEA 5 ± 0.7 10 ± 1.4 21 ± 2.0 32 ± 2.2 42 ± 2.2 13 ± 3.2
KCh J2.6 DHEA 100 ± 0.3 100 ± 0.5 81 ± 4.5 27 ± 3.8 6 ± 2.0 3 ± 1.1
7α-OH-DHEA 14 ± 3.1 38 ± 4.1 47 ± 1.4 34 ± 1.9
7β-OH-DHEA 5 ± 2.6 15 ± 3.1 28 ± 1.8 49 ± 2.1
KCh KW1.1 DHEA 100 ± 0.2 80 ± 2.1 53 ± 2.8 1 ± 0.6 1 ± 0.8
7α-OH-DHEA 12 ± 1.3 21 ± 2.6 68 ± 3.9 49 ± 1.3 34 ± 2.9 24 ± 2.7
7β-OH-DHEA 4 ± 0.7 8 ± 1.5 26 ± 0.6 39 ± 0.4 56 ± 3.1 56 ± 3.0
KCh KW1.2 DHEA 100 ± 0.3 95 ± 1.7 68 ± 3.9
7α-OH-DHEA 4 ± 0.6 22 ± 2.0 69 ± 0.7 50 ± 1.3 34 ± 2.6 23 ± 2.3
7β-OH-DHEA 1 ± 0.3 8 ± 1.9 27 ± 0.9 40 ± 0.5 56 ± 3.0 57 ± 4.4
KCh RJ1.1 DHEA 100 ± 0.1 100 ± 0.8 97 ± 0.7 27 ± 1.6 26 ± 3.3
7α-OH-DHEA 2 ± 0.2 38 ± 0.9 46 ± 1.7 51 ± 0.2 40 ± 3.0
7β-OH-DHEA 1 ± 0.3 16 ± 0.7 23 ± 2.8 46 ± 1.5 57 ± 2.4
  1. Biotransformation conditions: 100 mL of cultivation medium (3% glucose, 1% bacteriological peptone) in 300 mL Erlenmeyer flasks, 24 °C, 150 r/min for 7 day
  2. Data are the average of three independent experiments. Standard errors were indicated