Fig. 8

Characterization of batch and multi-component fed-batch cultivations with standard and reduced buffer concentration with E. coli BL21 (DE3) pRhotHi-2-EcFbFP. Left column: Depiction of a oxygen transfer rate (OTR) and ammonium; c pH in the culture medium; e osmolality; g Cell dry weight (CDW) of batch cultivations with Wilms-MOPS-mineral medium, with 200 and 50 mM MOPS buffer, 20 g/L glucose and inoculation OD₆₀₀: 0.5; right column: depiction of b oxygen transfer rate (OTR) and ammonium; d pH in the culture medium; f osmolality; h cell dry weight (CDW) of fed-batch cultivations with Wilms-MOPS-mineral medium with 200 mM MOPS buffer, 500 g/L initial glucose concentration, 40 g/L ammonium carbonate, blue dextran concentration: 1 g/L in the reservoir or 50 mM MOPS buffer with 500 g/L initial glucose concentration, 60 g/L ammonium carbonate, blue dextran concentration: 1 g/L in the reservoir, temperature: 37 °C, shaking frequency: 350 rpm, shaking diameter: 50 mm, initial culture medium volume: 10 mL, inoculation OD₆₀₀: 0.5, reservoir filling volume: 2 mL, dialysis membrane: Reichelt 10–20 kDa, membrane area: 18.1 mm²; strain: E. coli BL21 (DE3) pRhotHi-2-EcFbFP; Concentration values have been volume-corrected