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Table 3 Comparison of the molar yields (%) of products of ΔkstD1 and its recombinant strains using 5 g L−1 phytosterols

From: Identification, function, and application of 3-ketosteroid Δ1-dehydrogenase isozymes in Mycobacterium neoaurum DSM 1381 for the production of steroidic synthons

Strains

DSM 1381

ΔkstD1

HK1

HK2

HK3

PK1

PK2

PK3

ADD

0.99 ± 0.11

0.62 ± 0.17

1.01 ± 0.06

1.07 ± 0.27

0.49 ± 0.08

0.94 ± 0.16

1.38 ± 0.34

0.59 ± 0.22

AD

0.28 ± 0.04

0.8 ± 0.15

0.61 ± 0.15

0.3 ± 0.06

0.57 ± 0.09

0.31 ± 0

0.8 ± 0.22

0.52 ± 0.12

HPD

68.79 ± 6.76

1.63 ± 0.13

59.37 ± 5.62

72.19 ± 2.03

2.22 ± 0.21

67.96 ± 3.9

8.84 ± 0.26

1.8 ± 0.31

4HP

3.8 ± 0.63

67.01 ± 0.37

12.52 ± 1.68

3.47 ± 0.54

68.07 ± 1.55

5.63 ± 0.4

66.42 ± 2.72

68.82 ± 2.94

  1. DSM1381, M. neoaurum DSM 1381; ΔkstD1, mutant of M. neoaurum DSM 1381 deleted kstD1; HK1/HK2/HK3, ΔkstD1 harboring pMV306hsp-kstD1/pMV306hsp-kstD2/pMV306hsp-kstD3; PK1/PK2/PK3, ΔkstD1 harboring kstD1/kstD2/kstD3 expression plasmids under control of their own promoter