Fig. 1

Construction and characterization of regulated glucose-utilizing E. coli B0013-2021H. A Modified metabolic pathways of E. coli B0013 and its derivatives; B development flowchart of E. coli B0013-2021H and relatives; C growth characterization of E. coli B0013-2021H on glucose at different temperatures. The cells were cultivated on M9 plates with 5 g/l of glucose at 34 or 42 °C. a: E. coli B0013; b: B0013-1031H; c: B0013-2020H; d: B0013-2021H; D (a) ptsG mRNA fold change quantified by real-time quantitative PCR; (b) Expression level of phosphoenolpyruvate:glucose phosphotransferase in E. coli B0013-2021H at different incubation temperatures; E growth characterization of E. coli B0013-2021H and B0013-1031H. The cells grew in M9 medium supplemented with 5 g/l of glucose at 34, 37 or 42 °C and 200 rpm for 12 h. The cell densities were determined at 600 nm