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Fig. 1 | Microbial Cell Factories

Fig. 1

From: Metabolic engineering of Saccharomyces cerevisiae for efficient production of glucaric acid at high titer

Fig. 1

Biosynthetic pathway and integration strategy of miox4 and udh for over-producing glucaric acid in Saccharomyces cerevisiae. PCR-amplified products including miox4 and udh genes controlled by strong promoters were integrated into the delta locus with over-lapping HIS3 homology. INO1, myo-inositol-1-phosphate synthase gene; miox4, myo-inositol oxygenase gene; udh, uronate dehydrogenase gene; delta1 and delta2, multi-copy DNA sequence located in the reverse transcription transposon Ty of the chromosome DNA in S. cerevisiae. ADH1, ADH1 terminator; TEF1, TEF1 promoter; HIS, HIS3 selection marker gene; GPD1, GPD1 promoter; CYC1, CYC1 terminator

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Microbial Cell Factories

ISSN: 1475-2859

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