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Table 1 Kinetic parameters of IDI and IDI (L141H/Y195F/W256C)

From: Enhancement of the catalytic activity of Isopentenyl diphosphate isomerase (IDI) from Saccharomyces cerevisiae through random and site-directed mutagenesis

Enzyme Km (μM) Kcat (s−1) Kcat/Km (s−1 μM−1) Maximum enzyme activity (U/mg)
IDI 41.5 ± 0.39 8.27 ± 0.06 0.20 63.87 ± 1.09
IDI (L141H/Y195F/W256C) 37.6 ± 0.17 10.57 ± 0.13 0.28 161.60 ± 2.74