Fig. 5
From: Gene repression via multiplex gRNA strategy in Y. lipolytica
Repression of gfp via Multiplex CRISPRi system in Y. lipolytica. Regulation of gfp expression by dCpf1-Multi and dCas9-Multi system combined with multiplex gRNA targets. a Repression of gfp by dCpf1-Multi and dCas9-Multi system complexed with single gRNA, double gRNAs and triple gRNAs expressed by SCR1′-tRNAGly or SNR52′-tRNAGly promoter. b Characterization of the gfp gene’s expression level of each strain interfered by dCpf1-Multi and dCas9-Multi system. “+” means possess and “−” means not possess. The error bars (mean ± SD) were derived from triplicate experiments for each strain