Fig. 3
From: Gene repression via multiplex gRNA strategy in Y. lipolytica
Repression of gfp in Y. lipolytica by dCpf1-KRAB and dCas9-KRAB. a Schematic of dCpf1 fused to repressor domain KRAB and the dCpf1-KRAB expression system. b Schematic of dCas9 fused to repressor domain KRAB and the dCas9-KRAB expression system. c CRISPRi repression of gfp with dCpf1-KRAB complexed with ten gRNAs targeting different regions. The control (g0) shows fluorescence of the cells with dCpf1-KRAB protein but without the gRNA. d CRISPRi repression of gfp with dCas9-KRAB complexed with ten gRNAs targeting different regions. The control (g0) shows fluorescence of the cells with dCas9-KRAB protein but without the gRNA. The error bars (mean ± SD) were derived from triplicate experiments for each strain