Table 3 Primers used in this study
Primers | Sequences (5′-3′)a | Purposes |
|---|---|---|
P1 | CCGGAATTC ATGACTTTACCTGAATCAA | P1/P2: for PCR of ARO8 |
P2 | CTAGTCTAGAATAAAGTTGTACTCTTAATG | |
P3 | CCCGGCGCCTCGAGGTCGACGGTATC | P3/P4: for PCR of PGK1p + ARO8 |
P4 | CTAGGGCGCCATAAAGTTGTACTCTTAATG | |
P5 | TCCCCCCGGG ATGGCACCTGTTACAATTGAA | P5/P6: for PCR of ARO10 |
P6 | ACATGCATGC ACTCTGTGGTAGTGGTAAAA | |
P7 | TCCCCCCGGGATGTCTATTCCAGAAACTCA | P7/P8: for PCR of ADH2 |
P8 | CCGGAATTCTGTCTACAGTTTAGAGGAAT | |
P9 | CCCGACGTCTTTCGTCTTCAA | P9/P10: for PCR of UASTEF-RP-TEF1p +ARO10 |
P10 | CCCGACGTCACTCTGTGGTAGTGGTAAAA | |
P11 | CGGGGTACCCCGCAAGTAATACTTACAGCACT | P11/P12: for PCR of GDH2 |
P12 | GGAAGATCTTCCGCTGAGAAGAACATACAC | |
P13 | GGCATTGGCACTCATGACCT | P13/P14: for PCR of part of CUP1 + GDH2 P15/P16: for PCR of UASTEF-RG-ADH1p +ADH2 P17/P18: for PCR of ADH1p +GAP1 + ADH1t |
P14 P15 P16 P17 P18 | CTTTGGAGCATGGTAAGGATACC GCATGCCCGCGGCCACACA TGTCTACAGTTTAGAGGAAT TTATTCTTTCCTCTG GCCTCGACTGAAGGCTAGG | |
P19 | GTCATCACTATTGGTAACG | P19/P20: for qRT-PCR of ACT1 |
P20 | GGAGTTGTAAGTAGTTTGG | |
P21 | GCTGTTATCTTCCCTATTTCG | P21/P22: for qRT-PCR of GAP1 |
P22 | GTAGCACAACCAACCATT | |
P23 | GCCGCAACAGATGGATATTT | P23/P24: for qRT-PCR of ARO9 |
P24 | GCATAGGCGATGGTGAGTCT | |
P25 | TACCAAGATTATCCACGATT | P25/P26: for qRT-PCR of ARO8 |
P26 | AATGTGCCTCAACTAAGAT | |
P27 | CGCTTACAAGCGATTCACCA | P27/P28: for qRT-PCR of GDH2 |
P28 | GATGTCATCATCCTTAACAT | |
P29 | AACGCTCACATCAATGGT | P29/P30: for qRT-PCR of ARO10 |
P30 | ATGGTGCTCAGTTCTTGG | |
P31 | CTGCTGGTGGTCTAGGTTC | P31/P32: for qRT-PCR of ADH2 |
P32 | CCGAGCGAGGTAAACAATTC |