Reconstruction of metabolic module with improved promoter strength increases the productivity of 2-phenylethanol in Saccharomyces cerevisiae

Table 1 Activities of crucial enzymes in different yeast strains

Strains^a	Enzyme activities (IU mg⁻¹ protein)**
Strains^a	Aromatic aminotransferases	Phenylpyruvate decarboxylase
YS58-YEp	0.20 ± 0.01	0.06 ± 0.01
YS58(ARO8-58)	0.80 ± 0.02	0.07 ± 0.01
YS58(ARO10-58)	0.19 ± 0.02	0.29 ± 0.01
YS58(YEpAP58)	0.50 ± 0.01	0.14 ± 0.01
YS58(ARO8 + ARO10)	0.85 ± 0.03	0.36 ± 0.02
YS58(A8-A10-A2)	0.83 ± 0.02	0.37 ± 0.01
YS58(G1-A8-A10-A2)	0.98 ± 0.02	0.42 ± 0.02
YS58(G1-A8-A10-A2)-GDH	0.96 ± 0.03	0.43 ± 0.01

^aYS58-YEp, strain with control vector; YS58(ARO8-58), YS58(ARO10-58) and YS58(YEpAP58), strains with ARO8, ARO10 and GAP1 expression cassettes respectively; YS58(ARO8 + ARO10), ARO8 and ARO10 co-expression strain; YS58(A8-A10-A2), ARO8 + ARO10 + ADH2 co-expression strain; YS58(G1-A8-A10-A2), strain with GAP1 + ARO8 + ARO10 + ADH2 co-expression; YS58(G1-A8-A10-A2)-GDH, strain with GAP1 + ARO8 + ARO10 + ADH2 + GDH2 co-expression cassettes
** Significance of difference P < 0.01

ISSN: 1475-2859