Skip to main content
Fig. 3 | Microbial Cell Factories

Fig. 3

From: High-efficiency promoter-driven coordinated regulation of multiple metabolic nodes elevates lipid accumulation in the model microalga Phaeodactylum tricornutum

Fig. 3

Molecular analysis of engineered microalgae. a Schematic representation of the Pt211-D2G (DGAT2 and GPAT) construct. b Genomic PCR analysis of engineered strains showed the integration of Pt211-D2G construct. S1–S5: engineered strains, S6: WT, M: molecular marker. c, d qPCR analysis of DGAT2 (c) and GPAT (d) transcript levels. * and ** indicates the significant difference at p < 0.05 and p < 0.01 level, respectively. Each value represents mean ± SD (n = 3). e Western blot analysis. The anti-Flag and anti-Myc antibodies were used for the detection of DGAT2 and GPAT, respectively

Back to article page

Microbial Cell Factories

ISSN: 1475-2859

Contact us