Fig. 2From: Engineering CRISPR interference system in Klebsiella pneumoniae for attenuating lactic acid synthesisFluorescence assay of the engineered CRISPRi system in K. pneumoniae using EGFP as a reporter. NT, K. pneumoniae strain harboring CRISPRi vector without guide RNA; T1 and T2, K. pneumoniae strains harboring CRISPRi vectors targeting the different regions of promoter upstream of EGFP gene; aTc, anhydrotetracycline; “+”, aTc induction; “−”, without aTc. *P < 0.05; **P < 0.01Back to article page