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Fig. 2 | Microbial Cell Factories

Fig. 2

From: Engineering CRISPR interference system in Klebsiella pneumoniae for attenuating lactic acid synthesis

Fig. 2

Fluorescence assay of the engineered CRISPRi system in K. pneumoniae using EGFP as a reporter. NT, K. pneumoniae strain harboring CRISPRi vector without guide RNA; T1 and T2, K. pneumoniae strains harboring CRISPRi vectors targeting the different regions of promoter upstream of EGFP gene; aTc, anhydrotetracycline; “+”, aTc induction; “−”, without aTc. *P < 0.05; **P < 0.01

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