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Table 1 Characteristics and purification strategies of recombinant proteins

From: Lactococcus lactis provides an efficient platform for production of disulfide-rich recombinant proteins from Plasmodium falciparum

Antigena

Accession number

Target sequence

Cysteine (no)

Disorder tendencyb

Expression level

Capturing

Polishing

Mass (Da)

No carrier

With carrier

IMAC

Heat

IEC

Yield (mg/l)c

Calculated

Observedd

GPI-anchored

 MSP119k

PFI1475W

N1607–N1702

12

0

High

 

X

 

A

30

12.2

15

 MSP23D7

PFB0300C

A111–G238

0

100

High

 

X

 

A

20

13.7

40

 MSP2FC27

 

E143–G230

0

94

High

 

X

 

A

20

9.6

25

 Pf12

PFF0615C

K155–S323

6

0

Low

Med

 

X

C

10

20

20

 Pf38

PFE0395C

K139–I327

6

0

Low

Med

 

X

C

10

23

25

Peripherally associated

 nMSP33D7

PF10_0345

N21–L182

1

25

High

 

X

 

A

40

17.6

27

 cMSP33D7

PF10_0345

K183–H354

0

0

High

 

X

 

A

18

20

30

 nMSP3K1

PFU08851

N21–L208

0

48

High

 

X

 

A

35

21

37

 MSP6

PF10_346

Y17–N160

0

70

Med

 

X

 

A

10

17.5

23

 MSP3.3

PF10_0347

Q23–T227

0

48

Med

 

X

 

A

7

22.3

37

 MSP3.7

PF10_0352

K27–R213

0

100

Med

 

X

 

A

7

20.9

37

 GLURP-R0

AAA50613

T27–A500

0

99

High

 

X

 

A

75

54.3

90

 GLURP-R2

AAA50613

V705–1177

0

78

High

 

X

 

A

35

53

110

 SERA5

PF02_0072

K17–192/226–S382

8

40

Low

High

 

X

C

17

35.5

38

 Pf41

PFD0240C

S231–S378

6

0

Low

Med

 

X

C

7

18

18

 MSPDBL1

PF10_0348

K143–D443

13

0

Low

Med

 

X

C

8

37

37

 MSPDBL2

PF10_0355

K161–L457

12

0

Low

High

 

X

C

15

36.5

35

 MSPDBL1 (Leucine)

PF10_0348

D508–K697

0

69

Med

 

X

 

A

7

22

35

Micronemal/microneme

 EBA140RIII–V

MAL13P1.60

E746–T1045

0

93

High

 

X

 

A

16

32.9

40

 EBA140 RII

MAL13P1.60

Q146–K710

23

0

Low

Low

 

X

C

1,5

67.3

85

 GAMA

PF08_0008

N25–H337

7

10

Low

Med

 

X

A

5

36.8

45

 RIPR

PFC1045C

K279–D995

75

0

Low

Low

 

X

A

1

82.3

110

 SUB2

PF11_0381

K14–N681

2

29

Low

Med

 

X

A

5

78.3

90

Rhoptry

 PfRh2b

MAL13P1.176

L2790–K3184

0

91

Med

 

X

 

A

8

44.7

120

 PfRh2a

PF13_0198

H2874–S3060

0

26

High

 

X

 

A

13

22

25

 PfRh4.2

PFD1150C

L1275–I1451

0

11

Low

Med

X

 

C

2

21.5

21

 PfRh2-2030

PF13_0198

E2030–Q2528

1

0

Med

 

X

 

A

8

58

58

 RAMA

MAL7P1.208

I42–N786

0

100

Low

Med

X

 

A

7

90.6

120

 RALP-1

MAL7P1.119

N396–P634

0

47

Low

Med

X

 

A

10

27

37

 RON2

PF14_0495

I775–K958

0

49

Med

 

X

 

A

10

24

25

 RON4

PF11_0168

K25–L709

0

100

Low

Med

X

 

A

9

76

150

  1. A anion exchange (HiTrap Q HP); C cation exchange (Hitrap SP HP)
  2. aGene originate from 3D7 unless otherwise stated
  3. bDisorder prediction score was calculated by using IUPred [41]
  4. cThe levels of expressed protein was determined by BCA kit after final step purification. Expression levels are grouped into “low” (0.1–2 mg/l), “medium” (2–10 mg/l), and “high” (> 10 mg/l)
  5. dBy SDS-PAGE