Skip to main content

Table 1 Characteristics and purification strategies of recombinant proteins

From: Lactococcus lactis provides an efficient platform for production of disulfide-rich recombinant proteins from Plasmodium falciparum

Antigena Accession number Target sequence Cysteine (no) Disorder tendencyb Expression level Capturing Polishing Mass (Da)
No carrier With carrier IMAC Heat IEC Yield (mg/l)c Calculated Observedd
GPI-anchored
 MSP119k PFI1475W N1607–N1702 12 0 High   X   A 30 12.2 15
 MSP23D7 PFB0300C A111–G238 0 100 High   X   A 20 13.7 40
 MSP2FC27   E143–G230 0 94 High   X   A 20 9.6 25
 Pf12 PFF0615C K155–S323 6 0 Low Med   X C 10 20 20
 Pf38 PFE0395C K139–I327 6 0 Low Med   X C 10 23 25
Peripherally associated
 nMSP33D7 PF10_0345 N21–L182 1 25 High   X   A 40 17.6 27
 cMSP33D7 PF10_0345 K183–H354 0 0 High   X   A 18 20 30
 nMSP3K1 PFU08851 N21–L208 0 48 High   X   A 35 21 37
 MSP6 PF10_346 Y17–N160 0 70 Med   X   A 10 17.5 23
 MSP3.3 PF10_0347 Q23–T227 0 48 Med   X   A 7 22.3 37
 MSP3.7 PF10_0352 K27–R213 0 100 Med   X   A 7 20.9 37
 GLURP-R0 AAA50613 T27–A500 0 99 High   X   A 75 54.3 90
 GLURP-R2 AAA50613 V705–1177 0 78 High   X   A 35 53 110
 SERA5 PF02_0072 K17–192/226–S382 8 40 Low High   X C 17 35.5 38
 Pf41 PFD0240C S231–S378 6 0 Low Med   X C 7 18 18
 MSPDBL1 PF10_0348 K143–D443 13 0 Low Med   X C 8 37 37
 MSPDBL2 PF10_0355 K161–L457 12 0 Low High   X C 15 36.5 35
 MSPDBL1 (Leucine) PF10_0348 D508–K697 0 69 Med   X   A 7 22 35
Micronemal/microneme
 EBA140RIII–V MAL13P1.60 E746–T1045 0 93 High   X   A 16 32.9 40
 EBA140 RII MAL13P1.60 Q146–K710 23 0 Low Low   X C 1,5 67.3 85
 GAMA PF08_0008 N25–H337 7 10 Low Med   X A 5 36.8 45
 RIPR PFC1045C K279–D995 75 0 Low Low   X A 1 82.3 110
 SUB2 PF11_0381 K14–N681 2 29 Low Med   X A 5 78.3 90
Rhoptry
 PfRh2b MAL13P1.176 L2790–K3184 0 91 Med   X   A 8 44.7 120
 PfRh2a PF13_0198 H2874–S3060 0 26 High   X   A 13 22 25
 PfRh4.2 PFD1150C L1275–I1451 0 11 Low Med X   C 2 21.5 21
 PfRh2-2030 PF13_0198 E2030–Q2528 1 0 Med   X   A 8 58 58
 RAMA MAL7P1.208 I42–N786 0 100 Low Med X   A 7 90.6 120
 RALP-1 MAL7P1.119 N396–P634 0 47 Low Med X   A 10 27 37
 RON2 PF14_0495 I775–K958 0 49 Med   X   A 10 24 25
 RON4 PF11_0168 K25–L709 0 100 Low Med X   A 9 76 150
  1. A anion exchange (HiTrap Q HP); C cation exchange (Hitrap SP HP)
  2. aGene originate from 3D7 unless otherwise stated
  3. bDisorder prediction score was calculated by using IUPred [41]
  4. cThe levels of expressed protein was determined by BCA kit after final step purification. Expression levels are grouped into “low” (0.1–2 mg/l), “medium” (2–10 mg/l), and “high” (> 10 mg/l)
  5. dBy SDS-PAGE