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Table 4 Plasmids and strains used in this study

From: Metabolic engineering of a xylose pathway for biotechnological production of glycolate in Escherichia coli

Plasmids and strains

Description

Source

Plasmids

 pETDuet1

Amp r oriP BR322 lacI q P T7

Novagen

 pACYCDuet1

Cm r oriP 15A lacI q P T7

Novagen

 pLMe1

pETDuet1-ghrA

This study

 pLMe2

pETDuet1-yjhH-xylC-xdh

This study

 pLMe3

pETDuet1-yjhH-xylC-ghrA-xdh

This study

 pLMa1

pACYCDuet1-aceA

This study

 pLMa2

pACYCDuet1-aldA-yjhG

This study

 pLMa3

pACYCDuet1-aldA-aceA-yjhG

This study

Strains

 E. coli DH5α

F supE44 ΔlacU169 (ϕ80 lacZ ΔM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1

Invitrogen

 E. coli BL21(DE3)

F ompT gal dcm lon hsdSB (rB mB) λ(DE3)

Invitrogen

 JW3974

E. coli BW25113 aceB::kan

[39]

 JW2946

E. coli BW25113 glcD::kan

[39]

 JW3978

E. coli BW25113 iclR::kan

[39]

 JW2293

E. coli BW25113 ackA::kan

[39]

 Q2589

E. coli BL21(DE3) aceB::kan

This study

 Q2723

E. coli BL21(DE3) glcD::kan

This study

 Q2280

E. coli BL21(DE3) iclR::kan

This study

 Q1951

E. coli BL21(DE3) ackA::kan

This study

 Q2728

E. coli BL21(DE3)/pLMe1/pLMa1

This study

 Q2705

E. coli BL21(DE3) aceB::kan/pLMe1/pLMa1

This study

 Q2562

E. coli BL21(DE3)/pLMe2/pLMa2

This study

 Q2590

E. coli BL21(DE3) aceB::kan/pLMe2/pLMa2

This study

 Q2725

E. coli BL21(DE3)/pLMe2/pLMa3

This study

 Q2726

E. coli BL21(DE3)/pLMe3/pLMa2

This study

 Q2727

E. coli BL21(DE3) glcD::kan/pLMe2/pLMa2

This study

 Q2729

E. coli BL21(DE3)/pLMe3/pLMa3

This study

 Q2741

E. coli BL21(DE3) iclR::kan/pLMe2/pLMa2

This study

 Q2742

E. coli BL21(DE3) ackA::kan/pLMe2/pLMa2

This study