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Table 1 Production of glycolate after 24 h of IPTG induction under Erlenmeyer flasks cultivation on minimal medium with 15 g/L d-xylose

From: Metabolic engineering of a xylose pathway for biotechnological production of glycolate in Escherichia coli

Strains

Cell biomass (gCDW/L)

Cell yield (g/g xylose)

Glycolate concentration (g/L)

Glycolate yield (g/g xylose)

Acetate concentration (g/L)

Acetate yield (g/g xylose)

Q2728

1.05 ± 0.01

0.16 ± 0.07

0.28 ± 0.01

0.04 ± 0.01

0.66 ± 0.01

0.10 ± 0.02

Q2705

1.12 ± 0.04

0.14 ± 0.02

0.51 ± 0.11

0.05 ± 0.03

1.33 ± 0.15

0.16 ± 0.05

Q2562

1.03 ± 0.16

0.18 ± 0.09

2.53 ± 0.08

0.44 ± 0.10

0.95 ± 0.18

0.17 ± 0.04

Q2590

1.09 ± 0.13

0.24 ± 0.04

1.68 ± 0.09

0.37 ± 0.06

0.86 ± 0.13

0.19 ± 0.01

Q2725

0.99 ± 0.08

0.15 ± 0.11

2.65 ± 0.12

0.41 ± 0.14

0.83 ± 0.14

0.13 ± 0.06

Q2726

0.98 ± 0.15

0.16 ± 0.01

2.54 ± 0.03

0.41 ± 0.12

0.91 ± 0.11

0.15 ± 0.10

Q2727

1.02 ± 0.07

0.17 ± 0.05

2.61 ± 0.15

0.44 ± 0.08

0.84 ± 0.02

0.14 ± 0.07

Q2729

1.11 ± 0.05

0.18 ± 0.02

2.72 ± 0.11

0.43 ± 0.05

1.07 ± 0.07

0.17 ± 0.01

  1. Data were averages of triplicate experiments, and errors represent standard deviation