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Fig. 2 | Microbial Cell Factories

Fig. 2

From: Metabolic engineering of a xylose pathway for biotechnological production of glycolate in Escherichia coli

Fig. 2

Coomassie brilliant blue-stained SDS-PAGE analysis of recombinant proteins from different constructs. Lane M, protein molecular weight marker; lane 1, crude cells extracts from E. coli BL21(DE3)/pETDuet1/pACYCDuet1; lane 2, crude cells extracts from E. coli BL21(DE3) strain harboring pETDuet1-yjhH; lane 3, crude cells extracts from E. coli BL21(DE3) strain harboring pETDuet1-xylC-xdh; lane 4, crude cells extracts from E. coli BL21(DE3) strain harboring pACYCDuet1-aldA-yjhG; lane 5, crude cells extracts from E. coli BL21(DE3) strain harboring pETDuet1-ghrA and pACYCDuet1-aceA; lane 6, crude cells extracts from E. coli BL21(DE3) strain harboring pETDuet1-yjhH-xylC-ghrA-xdh and pACYCDuet1-aldA-aceA-yjhG. The position corresponding to the overexpressed genes were indicated by an arrow

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Microbial Cell Factories

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