Synthesis of avenanthramides using engineered Escherichia coli

Table 2 Conversion of HC and anthranilate into the corresponding N-HC-anthranilate, using strain HA-1

Substrate	Structure	HC-anthranilate^a (relative conversion rate %)
p-Coumaric acid		100
o-Coumaric acid		44.5 ± 2.7
Cinnamic acid		29.1 ± 3.6
Caffeic acid		18.5 ± 2.8
m-Coumaric acid		15.0 ± 1.2
3-Methoxycinnamic acid		12.3 ± 2.3
Ferulic acid		6.4 ± 0.8

^aE. coli strain HA-1 was used. Anthranilate and each HC (0.5 mM) was fed and the reaction was carried out at 30 °C for 3 h. The relative conversion rate was calculated by measuring the remaining amount of HC and the product. The best HC was considered as 100. The experiment was carried out in triplicate and the standard deviation was calculated

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