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Fig. 2 | Microbial Cell Factories

Fig. 2

From: Use of chitin and chitosan to produce new chitooligosaccharides by chitinase Chit42: enzymatic activity and structural basis of protein specificity

Fig. 2

PAGE analyses of Chit42 expressed in P. pastoris. Filtrates (5 μL) from yeasts grown in flask were evaluated after 0, 16, 24, 48, 72, 96 and 120 h of methanol induction (lane 1, 2, 3, 4, 5, 6, 7, respectively) using SDS-PAGE (a). Filtrate (20 μL) was revealed in situ after 96 h of induction (lane 1) (b). Filtrates from yeast grown in fed-batch and induced with methanol during 0, 48 h (0.5 μL; lane 1 and 2), 72 h (0.2 μL; lane 3) and 96 h (0.15 μL; lane 4) were analysed (c). Numbers on the left of panels indicate the positions of molecular mass standards (lane M) in kDa

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