Fig. 2From: Use of chitin and chitosan to produce new chitooligosaccharides by chitinase Chit42: enzymatic activity and structural basis of protein specificityPAGE analyses of Chit42 expressed in P. pastoris. Filtrates (5 μL) from yeasts grown in flask were evaluated after 0, 16, 24, 48, 72, 96 and 120 h of methanol induction (lane 1, 2, 3, 4, 5, 6, 7, respectively) using SDS-PAGE (a). Filtrate (20 μL) was revealed in situ after 96 h of induction (lane 1) (b). Filtrates from yeast grown in fed-batch and induced with methanol during 0, 48 h (0.5 μL; lane 1 and 2), 72 h (0.2 μL; lane 3) and 96 h (0.15 μL; lane 4) were analysed (c). Numbers on the left of panels indicate the positions of molecular mass standards (lane M) in kDaBack to article page