Development an effective system to expression recombinant protein in E. coli via comparison and optimization of signal peptides: Expression of Pseudomonas fluorescens BJ-10 thermostable lipase as case study

Table 2 Nucleotide sequence of signal peptide used in this study and the putative secretory pathway they follow

Strain name	Plasmid name	Putative secretion pathway	Signal peptide name	Signal peptide nucleotide sequence	Recombinant lipBJ10 name
BL21-NULL	pET-22b(+)	–	–	–	–
BL21-00	pET-SigPFL00	–	–	–	S(-)-lipBJ10
BL21-01	pET-SigPFL01	SEC	PelB	ATGAAATACCTGCTGCCGACCGCTGCTGCTGGTCTGCTGCTCCTCGCTGCCCAGCCGGCGATGGCC	PelB-lipBJ10
BL21-02	pET-SigPFL02	SRP	DsbA	ATGAAAAAAATTTGGCTGGCGCTGGCGGGCCTGGTGCTGGCGTTTAGCGCTAGCGCC	DsbA-lipBJ10
BL21-03	pET-SigPFL03	TAT + SEC	FhuD	ATGAGCGGCCTGCCGCTGATTAGCCGCCGCCGCCTGCTGACCGCGATGGCGCTGAGCCCGCTGCTGTGGCAGATGAACACCGCGCATGCC	FhuD-lipBJ10
BL21-04	pET-SigPFL04	TAT + SEC	MdoD	ATGGATCGCCGCCGCTTTATTAAAGGCAGCATGGCGATGGCGGCGGTGTGCGGCACCAGCGGCATTGCTAGCCTGTTTAGCCAGGCGGCGTTTGCC	MdoD-lipBJ10
BL21-05	pET-SigPFL05	SEC	OmpA	ATGAAAAAAACCGCGATTGCGATTGCGGTGGCGCTGGCGGGCTTTGCGACCGTGGCGCAGGCC	OmpA-lipBJ10
BL21-06	pET-SigPFL06	TAT + SEC	YcdO	ATGACCATTAACTTTCGCCGCAACGCGCTGCAGCTGAGCGTGGCGGCGCTGTTTAGCAGCGCGTTTATGGCGAACGCC	YcdO-lipBJ10

ISSN: 1475-2859