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Table 2 Nucleotide sequence of signal peptide used in this study and the putative secretory pathway they follow
| Strain name | Plasmid name | Putative secretion pathway | Signal peptide name | Signal peptide nucleotide sequence | Recombinant lipBJ10 name |
|---|---|---|---|---|---|
| BL21-NULL | pET-22b(+) | – | – | – | – |
| BL21-00 | pET-SigPFL00 | – | – | – | S(-)-lipBJ10 |
| BL21-01 | pET-SigPFL01 | SEC | PelB | ATGAAATACCTGCTGCCGACCGCTGCTGCTGGTCTGCTGCTCCTCGCTGCCCAGCCGGCGATGGCC | PelB-lipBJ10 |
| BL21-02 | pET-SigPFL02 | SRP | DsbA | ATGAAAAAAATTTGGCTGGCGCTGGCGGGCCTGGTGCTGGCGTTTAGCGCTAGCGCC | DsbA-lipBJ10 |
| BL21-03 | pET-SigPFL03 | TAT + SEC | FhuD | ATGAGCGGCCTGCCGCTGATTAGCCGCCGCCGCCTGCTGACCGCGATGGCGCTGAGCCCGCTGCTGTGGCAGATGAACACCGCGCATGCC | FhuD-lipBJ10 |
| BL21-04 | pET-SigPFL04 | TAT + SEC | MdoD | ATGGATCGCCGCCGCTTTATTAAAGGCAGCATGGCGATGGCGGCGGTGTGCGGCACCAGCGGCATTGCTAGCCTGTTTAGCCAGGCGGCGTTTGCC | MdoD-lipBJ10 |
| BL21-05 | pET-SigPFL05 | SEC | OmpA | ATGAAAAAAACCGCGATTGCGATTGCGGTGGCGCTGGCGGGCTTTGCGACCGTGGCGCAGGCC | OmpA-lipBJ10 |
| BL21-06 | pET-SigPFL06 | TAT + SEC | YcdO | ATGACCATTAACTTTCGCCGCAACGCGCTGCAGCTGAGCGTGGCGGCGCTGTTTAGCAGCGCGTTTATGGCGAACGCC | YcdO-lipBJ10 |
