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Table 2 Nucleotide sequence of signal peptide used in this study and the putative secretory pathway they follow

From: Development an effective system to expression recombinant protein in E. coli via comparison and optimization of signal peptides: Expression of Pseudomonas fluorescens BJ-10 thermostable lipase as case study

Strain name

Plasmid name

Putative secretion pathway

Signal peptide name

Signal peptide nucleotide sequence

Recombinant lipBJ10 name

BL21-NULL

pET-22b(+)

–

–

–

–

BL21-00

pET-SigPFL00

–

–

–

S(-)-lipBJ10

BL21-01

pET-SigPFL01

SEC

PelB

ATGAAATACCTGCTGCCGACCGCTGCTGCTGGTCTGCTGCTCCTCGCTGCCCAGCCGGCGATGGCC

PelB-lipBJ10

BL21-02

pET-SigPFL02

SRP

DsbA

ATGAAAAAAATTTGGCTGGCGCTGGCGGGCCTGGTGCTGGCGTTTAGCGCTAGCGCC

DsbA-lipBJ10

BL21-03

pET-SigPFL03

TAT + SEC

FhuD

ATGAGCGGCCTGCCGCTGATTAGCCGCCGCCGCCTGCTGACCGCGATGGCGCTGAGCCCGCTGCTGTGGCAGATGAACACCGCGCATGCC

FhuD-lipBJ10

BL21-04

pET-SigPFL04

TAT + SEC

MdoD

ATGGATCGCCGCCGCTTTATTAAAGGCAGCATGGCGATGGCGGCGGTGTGCGGCACCAGCGGCATTGCTAGCCTGTTTAGCCAGGCGGCGTTTGCC

MdoD-lipBJ10

BL21-05

pET-SigPFL05

SEC

OmpA

ATGAAAAAAACCGCGATTGCGATTGCGGTGGCGCTGGCGGGCTTTGCGACCGTGGCGCAGGCC

OmpA-lipBJ10

BL21-06

pET-SigPFL06

TAT + SEC

YcdO

ATGACCATTAACTTTCGCCGCAACGCGCTGCAGCTGAGCGTGGCGGCGCTGTTTAGCAGCGCGTTTATGGCGAACGCC

YcdO-lipBJ10