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Fig. 4 | Microbial Cell Factories

Fig. 4

From: Development an effective system to expression recombinant protein in E. coli via comparison and optimization of signal peptides: Expression of Pseudomonas fluorescens BJ-10 thermostable lipase as case study

Fig. 4

Comparison of Transmission Electron Microscopy of different Cells. The different strains, except BL21 (DE3), were cultivated in LB medium containing Amp (100 mg/ml) and grown at 37 °C until the value of OD600 reached 0.8, and then induced with 0.2 mM IPTG for 40 h at 20 °C. The BL21 (DE3) was cultivated in LB medium without Amp. Bar = 500 nm. a BL21 (DE3) (without plasmid). b BL21-NULL (with empty pET-22b(+)). c BL21-00 (with pET-SigPFL00). d BL21-01 (DE3) (with pET-SigPFL01). e BL21-02 (DE3) (with pET-SigPFL02). f BL21-03 (DE3) (with pET-SigPFL03). g BL21-04 (DE3) (with pET-SigPFL04). h BL21-05 (DE3) (with pET-SigPFL05). i BL21-06 (with pET-SigPFL06). White arrow: cytoplasmic inclusion bodies; Black arrow: periplasmic inclusion bodies

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