Table 2 Chromatographic analysis methods used for torulene and torularhodin
From: Torulene and torularhodin: “new” fungal carotenoids for industry?
Mobile phase composition | Elution type | Analytic column type | Detector | Literature |
|---|---|---|---|---|
Acetonitrile:isopropanol:ethyl acetate 4:4:2 (v/v/v) | Isocratic | C18 (Restek Ultra type, Restek) | Evaporative light scattering detector | [94] |
Acetonitrile:dichloromethane:methanol 7:2:1 (v/v/v) | Isocratic | Spherisorb ODS2 (Alltech Associates) | Refractometer detector | [95] |
Acetonitrile:tetrahydrofuran:water 5:3:1 (v/v/v) | Isocratic | C18 (µ-Bondapak type, Waters) | UV/Vis detector; 501 nm | [91] |
Methanol:acetonitrile 9:1 (v/v/v) | Isocratic | C18 (Novapak C type, Waters) | UV/Vis detector; 450 nm | [96] |
Acetone:water 95:5 (v/v) | Isocratic | RP-18 (LiChrospher 100 type, Merck) | UV/Vis detector; 450 nm | [97] |
Acetonitrile:methanol:methylene chloride 71:22:7 (v/v/v) | Isocratic | C18 (Waters type, Milford) | UV/Vis detector; 420–500 nm | [98] |
A: acetone with a 0.1% addition of trifluoroacetic acid (TFA) B: water with 0.1% TFA | Gradient | RP-18 (LiChrospher 100 type, Merck) | Diode-array detector | [27] |
A: methanol: ethyl acetate 1:1 (v/v) with a 0.05% addition of triethylamine and 0.1% BHT B: acetonitrile with a 0.05% addition of triethylamine and 0.1% BHT | Gradient | C18 (Supelcosil type, Sigma-Aldrich) | UV/Vis detector; 450 nm | [85] |
A: acetone B: water | Gradient | C18 (Cosmosil type, Nacalai Tesque) | Diode-array detector | [99] |