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Table 2 Chromatographic analysis methods used for torulene and torularhodin

From: Torulene and torularhodin: “new” fungal carotenoids for industry?

Mobile phase composition Elution type Analytic column type Detector Literature
Acetonitrile:isopropanol:ethyl acetate
4:4:2 (v/v/v)
Isocratic C18 (Restek Ultra type, Restek) Evaporative light scattering detector [94]
Acetonitrile:dichloromethane:methanol
7:2:1 (v/v/v)
Isocratic Spherisorb ODS2 (Alltech Associates) Refractometer detector [95]
Acetonitrile:tetrahydrofuran:water
5:3:1 (v/v/v)
Isocratic C18 (µ-Bondapak type, Waters) UV/Vis detector; 501 nm [91]
Methanol:acetonitrile
9:1 (v/v/v)
Isocratic C18 (Novapak C type, Waters) UV/Vis detector; 450 nm [96]
Acetone:water
95:5 (v/v)
Isocratic RP-18 (LiChrospher 100 type, Merck) UV/Vis detector; 450 nm [97]
Acetonitrile:methanol:methylene chloride
71:22:7 (v/v/v)
Isocratic C18 (Waters type, Milford) UV/Vis detector; 420–500 nm [98]
A: acetone with a 0.1% addition of trifluoroacetic acid (TFA)
B: water with 0.1% TFA
Gradient RP-18 (LiChrospher 100 type, Merck) Diode-array detector [27]
A: methanol: ethyl acetate 1:1 (v/v) with a 0.05% addition of triethylamine and 0.1% BHT
B: acetonitrile with a 0.05% addition of triethylamine and 0.1% BHT
Gradient C18 (Supelcosil type, Sigma-Aldrich) UV/Vis detector; 450 nm [85]
A: acetone
B: water
Gradient C18 (Cosmosil type, Nacalai Tesque) Diode-array detector [99]