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Fig. 2 | Microbial Cell Factories

Fig. 2

From: Comprehensive subcellular topologies of polypeptides in Streptomyces

Fig. 2

Overview of the topology annotation workflow. Protein sequences as derived after the re-annotation of S. lividans TK24 genome (Buche et al. in preparation) were used. The protein topology annotation in Uniprot was evaluated, revealing a large proportion of non-annotated proteins. De novo protein topology was assigned using prediction tools for topology or structural features followed by manual curation. Topology conflicts were manually curated. N: Nucleoid; r: Ribosome; A: Cytoplasmic; F1: Peripheral inner membrane protein facing the cytoplasm; B: Integral Membrane; F2 Peripheral inner membrane protein facing the periplasm; E: Inner Membrane Lipoprotein; P: Peptidoglycan binding; X: Extracellular; V: Extracellular vesicle; U: Uncharacterized

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