Fig. 5
From: TECS: a toxin expression control strategy as a tool for optimization of inducible promoters
Combining mutations in pBAD promoter and pO-oopRNA. a, b GFP fluorescence of plasmids p.1, p.6, p.8 from the entire promoter library and their variants with CGA mutation in BamHI site. Fluorescence was measured in uninduced (a) and induced (b) cells. Bars represent average of three independent experiments and error bars show standard variation. Asterisk above bar indicates significant difference in fluorescence between p.1CGA, p.1 and the wild type pBAD promoter. Significance was tested with t test and corresponding p values for comparisons are given. c, d Fluorescence of constructs in respect to the wild type pBAD promoter