Fig. 3
From: TECS: a toxin expression control strategy as a tool for optimization of inducible promoters
Introduction of the po-oopRNA sequence in the 5′ UTR. a Schematic representation of construct tested with TECS, which contains pO-oopRNA fragment from bacteriophage lambda. b Growth of selected variants of pO-oopRNA region. Eight individual colonies were tested for each variant. c Background expression of pO-oopRNA variants measured by GFP fluorescence intensity and presented as relative fluorescence units. d The activity of mutated pO promoters. Promoters were cloned into the pCh vector to drive the expression of mCherry gene