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Fig. 2 | Microbial Cell Factories

Fig. 2

From: TECS: a toxin expression control strategy as a tool for optimization of inducible promoters

Fig. 2

The concept and theoretical assumptions of TECS. a Schematic representation of the construct used to modify pBAD promoter which regulates expression of sacB gene. The BamHI site subjected to mutagenesis is circled. The underlined nucleotide in BamHI recognized sequence was subjected to mutagenesis. b Graphical representation of theoretical assumptions of TECS. c Growth of indicated mutants of the BamHI site. Two-fold dilutions of each mutant culture were plated on standard medium (LA), 5% sucrose (LA + Suc) and 5% sucrose plus 0.1% arabinose (LA + Suc + Ara). d Activity of mutated promoters measured with GFP fluorescence

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