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Table 3 Effect of PitA inactivation on His production by the strain KF37 [IS5.11::CmR-Ptac21-purA pitA−]

From: Specific features of l-histidine production by Escherichia coli concerned with feedback control of AICAR formation and inorganic phosphate/metal transport

Strain

pitA allele

OD540

His, g/L

His, %

KF37

pitA +

14.9 ± 0.4

3.3 ± 0.1

100

KF37 [IS5.11::CmR-Ptac21-purA, pitA+]

pitA +

16.1 ± 0.1

3.5 ± 0.1

106

KF37 [IS5.11::CmR-Ptac21-purA pitA−]

pitA −

15.6 ± 0.4

4.0 ± 0.1

121

  1. Average data from 8 independent experiments are represented