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Table 2 Specific activity of purified His6-tagged wild-type and feedback-resistant ATP-phosphoribosyltransferases and their inhibition by AMP and AICAR

From: Specific features of l-histidine production by Escherichia coli concerned with feedback control of AICAR formation and inorganic phosphate/metal transport

Enzyme ATP-phosphoribosyltransferase activity, µmol/min/mg Inhibition, %
AMP AICAR AMP AICAR
1 mM 20 mM 0.5 mM 1 mM 1 mM 20 mM 0.5 mM 1 mM
HT-HisG 184 ± 9 195 ± 1 120 ± 15 151 ± 9 120 ± 5 0 35 18 35
HT-HisGE271K 120 ± 1 129 ± 12 nm 67 ± 4 58 ± 8 0 nm 44 52
  1. Average data of 3 independent experiments are represented
  2. nm not measured