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Table 2 Specific activity of purified His6-tagged wild-type and feedback-resistant ATP-phosphoribosyltransferases and their inhibition by AMP and AICAR

From: Specific features of l-histidine production by Escherichia coli concerned with feedback control of AICAR formation and inorganic phosphate/metal transport

Enzyme

ATP-phosphoribosyltransferase activity, µmol/min/mg

Inhibition, %

–

AMP

AICAR

AMP

AICAR

–

1 mM

20 mM

0.5 mM

1 mM

1 mM

20 mM

0.5 mM

1 mM

HT-HisG

184 ± 9

195 ± 1

120 ± 15

151 ± 9

120 ± 5

0

35

18

35

HT-HisGE271K

120 ± 1

129 ± 12

nm

67 ± 4

58 ± 8

0

nm

44

52

  1. Average data of 3 independent experiments are represented
  2. nm not measured