|
Strains
|
|
MG1655
|
Escherichia coli K-12 wild-type
|
VKPMa B-6195
|
|
BL21(DE3)
|
E. coli B F− ompT gal dcm lon hsdS
B
(r
−
B
m
−
B
) λ(DE3 [lacI PlacUV5-T7gene1 ind1 sam7 nin5]) [malB+]K–12 (λS)
|
[32]
|
|
KF37
|
MG1655+-[ΔpurR P
his
-ΔhisL′ hisGE271KDCBHAFI]
|
[13]
|
|
MG1655 [ΔpurR::CmR]
|
MG1655 wild-type E. coli K-12, but ΔpurR::λattR-cat-λattL
|
Laboratory collection
|
|
BW25113 [ΔpurH::KmR]
|
lacIq rrnBT14 ΔlacZWJ16 hsdR514 ΔaraBADAH33 ΔrhaBADLD78, ΔpurH::FRT-kan-FRT
|
[33]
|
|
MG1655 [Δ(φ80-attB)]
|
MG1655 with deleted native (φ80-attB) site
|
[34]
|
|
MG1655 [Δ(φ80-attB) IS 5.11::φ80-attB]
|
MG1655 with deleted native φ80 attB site and reconstruction of attB site in IS 5.11 locus
|
[34]
|
|
MG1655 [Δ(φ80-attB) IS 5.11::CmR-Ptac21-purA]
|
MG1655-[Δ(φ80-attB) IS 5.11::φ80-attB], but IS 5.11::(λ-attB) Ptac21-purA
|
This study
|
|
MG1655 [CmR-PL-purH]
|
MG1655, but λattR-cat-λattL-PL-purH
|
This study
|
|
MG1655 [ΔpitA::KmR]
|
MG1655, but ΔpitA::λattR-kan-λattL
|
This study
|
|
MG1655 [CmR-PLtac-pitA]
|
MG1655, but λattR-cat-λattL-PLtac-pitA
|
This study
|
|
MG1655 [ΔpurR::CmR ΔpurH:: KmR]
|
MG1655, but ΔpurR::λattR-cat-λattL and ΔpurR::FRT-kan-FRT
|
This study
|
|
KF37 [ΔpitA::KmR]
|
KF37, but ΔpitA::λattR-kan-λattL
|
This study
|
|
KF37 [IS5.11::CmR-Ptac21-purA pitA−
|
KF37, but IS 5.11:: λattR-cat-λattL Ptac21-purA pitA−
|
This study
|
|
KF37 [IS5.11::CmR-Ptac21-purA pitA+]
|
KF37, but IS 5.11::λattR-cat-λattL Ptac21-purA pitA+
|
This study
|
|
EA79
|
KF37, but IS 5.11::(λ-attB) Ptac21-purA pitA−
|
This study
|
|
EA83
|
EA79, but (λ-attB) PL-purH
|
This study
|
|
CC118 λpir+
|
Host strain for maintenance of pir-dependent recombinant plasmids
|
[35]
|
|
MG1655 [Δ(φ80-attB) ∆yibH::φ80-attB]
|
MG1655 with deleted native φ80 attB site and reconstruction of attB site instead of yibH locus
|
This study
|
|
MG1655 [∆yibH::TcR-phoBDBD]
|
MG1655 [Δ(φ80-attB) ∆yibH::φ80-attB], but ∆yibH::λattR- tetAR-λattL-phoBDBD
|
This study
|
|
MG1655 [∆yibH::KmR-PlacUV5-phoBDBD]
|
MG1655 [Δ(φ80-attB) ∆yibH:: λattR-tetAR-λattL -phoBDBD], but ∆yibH::λattR-kan-λattL-PlacUV5-phoBDBD
|
This study
|
|
KF37 [∆yibH::TcR-phoBDBD]
|
KF37, but ∆yibH::λattR- tetAR-λattL-phoBDBD
|
This study
|
|
KF37 [∆yibH::KmR-PlacUV5-phoBDBD]
|
KF37, but ∆yibH::λattR-kan-λattL-PlacUV5-phoBDBD
|
This study
|
|
Plasmids
|
|
pKD46
|
oriR101, repA101ts, araC, P
araB
-[γ, β, exo of phage λ], ApR; used as a donor of λRed-genes to provide λRed-dependent recombination
|
[36]
|
|
pMWts-λInt/Xis
|
oriR101, repA101ts, λcIts857, λPR→λxis-int, ApR; used as a helper plasmid for thermoinducible expression of the λ xis-int genes
|
[34]
|
|
pAH123
|
oriR101, repA101ts, λcIts857, λPR→φ80-int, ApR; used as a helper plasmid for thermoinducible expression of the φ80-int gene
|
[37]; GenBank accession number AY048726
|
|
pET15b
|
ApR, pBR322 origin, P
lacI
-lacI, and T7 promoter/O
lac
, T7 transcription start, His6-Tag coding sequence, T7 terminator
|
Novagen
|
|
pMW118-KmR
|
oriR101, repA, MCS, ApR, λattR-kan-λattL—donor of λXis/Int-excisable KmR marker
|
[38]
|
|
pET15b-hisG
|
ApR, pET15b containing hisG gene
|
This study
|
|
pET15b-hisGE271K
|
ApR, pET15b containing the mutant hisGE271K gene
|
This study
|
|
pAH162-TcR-2Ter
|
attP phi80, pAH162, λattL-tetA-tetR-λattR
|
[34]; Gene Bank accession number AY048738
|
|
pMW119-P
lac
-lacI
|
ApR, low-copy-number vector pMW119, containing P
lac
-lacI
|
Laboratory collection
|
|
pMW119-P
lac
-lacI-purA
|
ApR, low-copy-number vector pMW119, containing P
lac
-lacI-purA
|
This study
|
|
pAH162-TcR-2Ter-purA
|
oriRγ, φ80-attP, λattL-tetA-tetR-λattR, purA
|
This study
|
|
pML-PlacUV5-lacI
|
PlacUV5-lacI, rep(pMB1), ApR, CmR
|
[39]
|
|
pAH162-TcR-2Ter-phoBDBD
|
oriRγ, φ80-attP, λattL-tetA-tetR-λattR, promoter-less phoBDBD
|
This study
|
