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Table 1 E. coli strains and plasmids used in this study

From: Specific features of l-histidine production by Escherichia coli concerned with feedback control of AICAR formation and inorganic phosphate/metal transport

Strain or plasmid Description Source
Strains
 MG1655 Escherichia coli K-12 wild-type VKPMa B-6195
 BL21(DE3) E. coli B F ompT gal dcm lon hsdS B (r B m B ) λ(DE3 [lacI PlacUV5-T7gene1 ind1 sam7 nin5]) [malB+]K–12S) [32]
 KF37 MG1655+-[ΔpurR P his hisLhisGE271KDCBHAFI] [13]
 MG1655 [ΔpurR::CmR] MG1655 wild-type E. coli K-12, but ΔpurR::λattR-catattL Laboratory collection
 BW25113 [ΔpurH::KmR] lacIq rrnBT14 ΔlacZWJ16 hsdR514 ΔaraBADAH33 ΔrhaBADLD78, ΔpurH::FRT-kan-FRT [33]
 MG1655 [Δ(φ80-attB)] MG1655 with deleted native (φ80-attB) site [34]
 MG1655 [Δ(φ80-attB) IS 5.11::φ80-attB] MG1655 with deleted native φ80 attB site and reconstruction of attB site in IS 5.11 locus [34]
 MG1655 [Δ(φ80-attB) IS 5.11::CmR-Ptac21-purA] MG1655-[Δ(φ80-attB) IS 5.11::φ80-attB], but IS 5.11::(λ-attB) Ptac21-purA This study
 MG1655 [CmR-PL-purH] MG1655, but λattR-catattL-PL-purH This study
 MG1655 [ΔpitA::KmR] MG1655, but ΔpitA::λattR-kanattL This study
 MG1655 [CmR-PLtac-pitA] MG1655, but λattR-catattL-PLtac-pitA This study
 MG1655 [ΔpurR::CmR ΔpurH:: KmR] MG1655, but ΔpurR::λattR-catattL and ΔpurR::FRT-kan-FRT This study
 KF37 [ΔpitA::KmR] KF37, but ΔpitA::λattR-kanattL This study
 KF37 [IS5.11::CmR-Ptac21-purA pitA KF37, but IS 5.11:: λattR-catattL Ptac21-purA pitA This study
 KF37 [IS5.11::CmR-Ptac21-purA pitA+] KF37, but IS 5.11::λattR-catattL Ptac21-purA pitA+ This study
 EA79 KF37, but IS 5.11::(λ-attB) Ptac21-purA pitA This study
 EA83 EA79, but (λ-attB) PL-purH This study
 CC118 λpir+ Host strain for maintenance of pir-dependent recombinant plasmids [35]
 MG1655 [Δ(φ80-attB)  ∆yibH::φ80-attB] MG1655 with deleted native φ80 attB site and reconstruction of attB site instead of yibH locus This study
 MG1655 [∆yibH::TcR-phoBDBD] MG1655 [Δ(φ80-attB) ∆yibH::φ80-attB], but ∆yibH::λattR- tetARattL-phoBDBD This study
 MG1655 [∆yibH::KmR-PlacUV5-phoBDBD] MG1655 [Δ(φ80-attB) ∆yibH:: λattR-tetARattL -phoBDBD], but ∆yibH::λattR-kanattL-PlacUV5-phoBDBD This study
 KF37 [∆yibH::TcR-phoBDBD] KF37, but ∆yibH::λattR- tetARattL-phoBDBD This study
 KF37 [∆yibH::KmR-PlacUV5-phoBDBD] KF37, but ∆yibH::λattR-kanattL-PlacUV5-phoBDBD This study
Plasmids
 pKD46 oriR101, repA101ts, araC, P araB -[γ, β, exo of phage λ], ApR; used as a donor of λRed-genes to provide λRed-dependent recombination [36]
 pMWts-λInt/Xis oriR101, repA101ts, λcIts857, λPR→λxis-int, ApR; used as a helper plasmid for thermoinducible expression of the λ xis-int genes [34]
 pAH123 oriR101, repA101ts, λcIts857, λPR→φ80-int, ApR; used as a helper plasmid for thermoinducible expression of the φ80-int gene [37]; GenBank accession number AY048726
 pET15b ApR, pBR322 origin, P lacI -lacI, and T7 promoter/O lac , T7 transcription start, His6-Tag coding sequence, T7 terminator Novagen
 pMW118-KmR oriR101, repA, MCS, ApR, λattR-kanattL—donor of λXis/Int-excisable KmR marker [38]
 pET15b-hisG ApR, pET15b containing hisG gene This study
 pET15b-hisGE271K ApR, pET15b containing the mutant hisGE271K gene This study
 pAH162-TcR-2Ter attP phi80, pAH162, λattL-tetA-tetRattR [34]; Gene Bank accession number AY048738
 pMW119-P lac -lacI ApR, low-copy-number vector pMW119, containing P lac -lacI Laboratory collection
 pMW119-P lac -lacI-purA ApR, low-copy-number vector pMW119, containing P lac -lacI-purA This study
 pAH162-TcR-2Ter-purA oriRγ, φ80-attP, λattL-tetA-tetRattR, purA This study
 pML-PlacUV5-lacI PlacUV5-lacI, rep(pMB1), ApR, CmR [39]
 pAH162-TcR-2Ter-phoBDBD oriRγ, φ80-attP, λattL-tetA-tetRattR, promoter-less phoBDBD This study
  1. ApR ampicillin resistance, CmR chloramphenicol resistance, TcR tetracycline resistance
  2. aVKPM Russian National Collection of Industrial Microorganisms