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Table 1 E. coli strains and plasmids used in this study
| Strain or plasmid | Description | Source |
|---|---|---|
| Strains | ||
| MG1655 | Escherichia coli K-12 wild-type | VKPMa B-6195 |
| BL21(DE3) | E. coli B F− ompT gal dcm lon hsdS B (r B − m B − ) λ(DE3 [lacI PlacUV5-T7gene1 ind1 sam7 nin5]) [malB+]K–12 (λS) | [32] |
| KF37 | MG1655+-[ΔpurR P his -ΔhisL′ hisGE271KDCBHAFI] | [13] |
| MG1655 [ΔpurR::CmR] | MG1655 wild-type E. coli K-12, but ΔpurR::λattR-cat-λattL | Laboratory collection |
| BW25113 [ΔpurH::KmR] | lacIq rrnBT14 ΔlacZWJ16 hsdR514 ΔaraBADAH33 ΔrhaBADLD78, ΔpurH::FRT-kan-FRT | [33] |
| MG1655 [Δ(φ80-attB)] | MG1655 with deleted native (φ80-attB) site | [34] |
| MG1655 [Δ(φ80-attB) IS 5.11::φ80-attB] | MG1655 with deleted native φ80 attB site and reconstruction of attB site in IS 5.11 locus | [34] |
| MG1655 [Δ(φ80-attB) IS 5.11::CmR-Ptac21-purA] | MG1655-[Δ(φ80-attB) IS 5.11::φ80-attB], but IS 5.11::(λ-attB) Ptac21-purA | This study |
| MG1655 [CmR-PL-purH] | MG1655, but λattR-cat-λattL-PL-purH | This study |
| MG1655 [ΔpitA::KmR] | MG1655, but ΔpitA::λattR-kan-λattL | This study |
| MG1655 [CmR-PLtac-pitA] | MG1655, but λattR-cat-λattL-PLtac-pitA | This study |
| MG1655 [ΔpurR::CmR ΔpurH:: KmR] | MG1655, but ΔpurR::λattR-cat-λattL and ΔpurR::FRT-kan-FRT | This study |
| KF37 [ΔpitA::KmR] | KF37, but ΔpitA::λattR-kan-λattL | This study |
| KF37 [IS5.11::CmR-Ptac21-purA pitA− | KF37, but IS 5.11:: λattR-cat-λattL Ptac21-purA pitA− | This study |
| KF37 [IS5.11::CmR-Ptac21-purA pitA+] | KF37, but IS 5.11::λattR-cat-λattL Ptac21-purA pitA+ | This study |
| EA79 | KF37, but IS 5.11::(λ-attB) Ptac21-purA pitA− | This study |
| EA83 | EA79, but (λ-attB) PL-purH | This study |
| CC118 λpir+ | Host strain for maintenance of pir-dependent recombinant plasmids | [35] |
| MG1655 [Δ(φ80-attB) ∆yibH::φ80-attB] | MG1655 with deleted native φ80 attB site and reconstruction of attB site instead of yibH locus | This study |
| MG1655 [∆yibH::TcR-phoBDBD] | MG1655 [Δ(φ80-attB) ∆yibH::φ80-attB], but ∆yibH::λattR- tetAR-λattL-phoBDBD | This study |
| MG1655 [∆yibH::KmR-PlacUV5-phoBDBD] | MG1655 [Δ(φ80-attB) ∆yibH:: λattR-tetAR-λattL -phoBDBD], but ∆yibH::λattR-kan-λattL-PlacUV5-phoBDBD | This study |
| KF37 [∆yibH::TcR-phoBDBD] | KF37, but ∆yibH::λattR- tetAR-λattL-phoBDBD | This study |
| KF37 [∆yibH::KmR-PlacUV5-phoBDBD] | KF37, but ∆yibH::λattR-kan-λattL-PlacUV5-phoBDBD | This study |
| Plasmids | ||
| pKD46 | oriR101, repA101ts, araC, P araB -[γ, β, exo of phage λ], ApR; used as a donor of λRed-genes to provide λRed-dependent recombination | [36] |
| pMWts-λInt/Xis | oriR101, repA101ts, λcIts857, λPR→λxis-int, ApR; used as a helper plasmid for thermoinducible expression of the λ xis-int genes | [34] |
| pAH123 | oriR101, repA101ts, λcIts857, λPR→φ80-int, ApR; used as a helper plasmid for thermoinducible expression of the φ80-int gene | [37]; GenBank accession number AY048726 |
| pET15b | ApR, pBR322 origin, P lacI -lacI, and T7 promoter/O lac , T7 transcription start, His6-Tag coding sequence, T7 terminator | Novagen |
| pMW118-KmR | oriR101, repA, MCS, ApR, λattR-kan-λattL—donor of λXis/Int-excisable KmR marker | [38] |
| pET15b-hisG | ApR, pET15b containing hisG gene | This study |
| pET15b-hisGE271K | ApR, pET15b containing the mutant hisGE271K gene | This study |
| pAH162-TcR-2Ter | attP phi80, pAH162, λattL-tetA-tetR-λattR | [34]; Gene Bank accession number AY048738 |
| pMW119-P lac -lacI | ApR, low-copy-number vector pMW119, containing P lac -lacI | Laboratory collection |
| pMW119-P lac -lacI-purA | ApR, low-copy-number vector pMW119, containing P lac -lacI-purA | This study |
| pAH162-TcR-2Ter-purA | oriRγ, φ80-attP, λattL-tetA-tetR-λattR, purA | This study |
| pML-PlacUV5-lacI | PlacUV5-lacI, rep(pMB1), ApR, CmR | [39] |
| pAH162-TcR-2Ter-phoBDBD | oriRγ, φ80-attP, λattL-tetA-tetR-λattR, promoter-less phoBDBD | This study |
