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Table 4 Experimental CCD on dodecane degradation by strain ADL36 with six centre points showing observed and predicted values

From: Evaluation of conventional and response surface level optimisation of n-dodecane (n-C12) mineralisation by psychrotolerant strains isolated from pristine soil at Southern Victoria Island, Antarctica

Run order

X 1

X 2

X 3

X 4

Dodecane mineralisation (%)

Experimental value

Predicted value

1

7.25

40.00

2.50

1.00

18.26

25.81

2

8.00

30.00

4.00

2.00

17.05

18.66

3

7.25

20.00

2.50

− 1.00

95.67

91.93

4

7.25

20.00

2.50

1.00

93.34

88.76

5

7.25

20.00

2.50

1.00

93.01

88.76

6

8.75

20.00

2.50

1.00

37.27

41.80

7

8.00

10.00

1.00

0.00

14.16

15.72

8

8.00

10.00

4.00

2.00

3.61

6.12

9

8.00

30.00

1.00

0.00

57.00

51.69

10

7.25

20.00

2.50

1.00

90.12

88.76

11

7.25

20.00

2.50

1.00

87.67

88.76

12

7.25

0.00

2.50

1.00

0.70

− 6.23

13

5.75

20.00

2.50

1.00

70.00

66.09

14

6.50

10.00

4.00

0.00

34.34

43.74

15

6.50

30.00

1.00

0.00

69.27

67.24

16

8.00

30.00

4.00

0.00

71.50

72.04

17

6.50

10.00

4.00

2.00

10.63

14.85

18

7.25

20.00

2.50

1.00

87.45

88.76

19

6.50

10.00

1.00

0.00

50.46

47.75

20

7.25

20.00

5.50

1.00

21.71

10.11

21

6.50

30.00

4.00

0.00

76.81

79.80

22

6.50

30.00

4.00

2.00

12.01

10.92

23

8.00

30.00

1.00

2.00

25.70

16.78

24

8.00

10.00

1.00

2.00

24.89

20.80

25

6.50

30.00

1.00

2.00

20.35

16.83

26

8.00

10.00

4.00

0.00

17.09

19.50

27

7.25

20.00

2.50

3.00

23.78

28.13

28

7.25

20.00

2.50

1.00

81.00

88.76

29

7.25

20.00

− 0.50

1.00

0.00

12.23

30

6.50

10.00

1.00

2.00

37.38

37.32

  1. CCD data are expressed as mean ± SD, n = 3
  2. X 1 : pH; X 2 : Temperature (°C); X 3 : Diesel concentration (% v/v); X 4 : NaCl concentration (% w/v)