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Table 2 Experimental CCD on n-dodecane degradation by strain ADL15 with six centre points showing observed and predicted values

From: Evaluation of conventional and response surface level optimisation of n-dodecane (n-C12) mineralisation by psychrotolerant strains isolated from pristine soil at Southern Victoria Island, Antarctica

Run order

X 1

X 2

X 3

X 4

Dodecane mineralisation (%)

Experimental value

Predicted value

1

7.25

40.00

2.50

1.00

0.83

4.86

2

8.00

30.00

4.00

2.00

0.71

0.82

3

7.25

20.00

2.50

− 1.00

34.77

32.88

4

7.25

20.00

2.50

1.00

33.34

30.73

5

7.25

20.00

2.50

1.00

32.01

30.73

6

8.75

20.00

2.50

1.00

17.27

16.70

7

8.00

10.00

1.00

0.00

14.42

12.79

8

8.00

10.00

4.00

2.00

0.04

0.99

9

8.00

30.00

1.00

0.00

26.50

24.43

10

7.25

20.00

2.50

1.00

31.67

30.73

11

7.25

20.00

2.50

1.00

30.45

30.73

12

7.25

0.00

2.50

1.00

0.01

− 3.17

13

5.75

20.00

2.50

1.00

20.00

21.42

14

6.50

10.00

4.00

0.00

4.34

6.22

15

6.50

30.00

1.00

0.00

29.81

26.77

16

8.00

30.00

4.00

0.00

19.00

21.46

17

6.50

10.00

4.00

2.00

0.06

3.37

18

7.25

20.00

2.50

1.00

30.12

30.73

19

6.50

10.00

1.00

0.00

17.46

18.57

20

7.25

20.00

5.50

1.00

1.71

− 4.94

21

6.50

30.00

4.00

0.00

19.27

19.77

22

6.50

30.00

4.00

2.00

0.23

− 0.23

23

8.00

30.00

1.00

2.00

4.89

0.92

24

8.00

10.00

1.00

2.00

5.70

6.43

25

6.50

30.00

1.00

2.00

6.35

3.89

26

8.00

10.00

4.00

0.00

0.80

4.48

27

7.25

20.00

2.50

3.00

3.78

6.51

28

7.25

20.00

2.50

1.00

26.83

30.73

29

7.25

20.00

− 0.50

1.00

0.00

7.50

30

6.50

10.00

1.00

2.00

17.38

12.84

  1. CCD data are shown as mean ± SD, n = 3
  2. X 1 : pH; X 2 : temperature (°C); X 3 : diesel concentration (% v/v); X 4 : NaCl concentration (% w/v)