Genome editing and transcriptional repression in Pseudomonas putida KT2440 via the type II CRISPR system

Table 2 Comparison of different genetic editing tools in Pseudomonas

Number	Method	Time spent	Scarless	Markerless	Insertion	Exact locus	Mutation efficiency	Convenient continual genome editing	Efficient plasmid curing	References
1	This work	5 days	Yes	Yes	Yes	Yes	70–100%	Yes	Yes
2	CRISPR–Cas9 Ssr	4–5 days	Yes	Yes	ND	Yes	13–93%	No	No	[37]
3	I-SceI	5–6 days	Yes	Yes	ND	Yes	14–84%	No	No	[14]
4	Tn5 FLP-FRI	2 weeks	No	No	No	No	ND	No	No	[17]
5	Tn5/Tn7	l week	No	No	Yes	No	ND	No	No	[15, 16]
6	Flp/FRT SacB	5–6 days	No	Yes	ND	Yes	ND	No	No	[9]
7	λ-Red SacB	4–5 days	Yes	Yes	ND	Yes	88–98%	No	No	[12]
8	Cre-lox λ-Red	4–6 days	No	Yes	ND	Yes	70–100%	No	No	[13]
9	pyrF	2 weeks	No	No	ND	Yes	ND	No	No	[6]
10	upp	5–6 days	Yes	Yes	Yes	Yes	10–40%	No	No	[7]
11	Red/ET	4–5 days	No	No	Yes	Yes	ND	No	No	[11]

ISSN: 1475-2859