Fig. 6From: Genome editing and transcriptional repression in Pseudomonas putida KT2440 via the type II CRISPR systemCRISPR–Cpf1 mediated genome editing in the Pseudomonas putida KT2440. a Overview of the genome editing by CRISPR–Cpf1 in P. putida KT2440. b The schematic showing the design of identification primers to confirm genome editing in KT2440. c Agarose gel electrophoresis and the result of DNA sequencing show that pCpf1-RK2K enables gene deletion in KT2440Back to article page