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Fig. 3 | Microbial Cell Factories

Fig. 3

From: A synbio approach for selection of highly expressed gene variants in Gram-positive bacteria

Fig. 3

Antibiotic selection of maximal protein production in a Gram-positive bacterium. a B. subtilis TIR libraries expressing gfp were grown on agar plates with different chloramphenicol concentrations. GFP production levels on the different antibiotic concentrations were assayed by exposing agar plates to long-wave UV light. Five individual colonies were randomly picked from these plates and assayed for expression levels assessed by fluorescence per cell density in a microplate reader (b, c)

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