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Fig. 2 | Microbial Cell Factories

Fig. 2

From: A synbio approach for selection of highly expressed gene variants in Gram-positive bacteria

Fig. 2

A part of the translation initiation region (TIR) affects expression in Bacillus subtilis and Lactococcus lactis. a TIR libraries (NNNNNNATGN*N*N*N*N*N*, see main text for further details) were constructed by PCR, transformed into the host strains and individual library clones were grown to asses the expression level ranges in B. subtilis and L. lactis. Fluorescence normalized by cell density was determined for 96 library clones for B. subtilis (b) and L. lactis (c). The B. subtilis library was expressed from the genome whereas the L. lactis library was expressed from a plasmid

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