Table 2 Primers were used for random and site directed mutation

1

Lip-F: CGCGGATCCATGGCACTGGAAAAGGGT (with BamH I restriction site underlined)

2

Lip-R: CCGCTCGAGTCACTCTCGCCCGGCA (with XhoI restriction site underlined)

3

Lip-V29A-F: GCCAGACAATTGAGGCACTACGAGCA

4

Lip-V29A-R: GCCTCAATTGTCTGGCGACGCGATG

5

Lip-Y193C-F: CCAGTCTTGAAAGCTGTACCCTCAAT

6

Lip-Y193C-R: CAGCTTTCAAGACTGGCCGTAGCGT