Fig. 2
a Substrate specificity of purified Lip. The activity towards C2 as 100%. b Effect of temperature on the activity of Lip and mutants. The activity at 40 °C as 100%. c Effect of pH on the activity of Lip and mutants. Activity of wild type, V29A, Y193C and V29A/Y193C at pH 8.5 and 9 as 100%. d Thermo-stability of Lip and Y193C. The residual activity was measured at 40 °C by collecting enzymes every 30 min. The specific activity without incubation was defined as 100%