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Fig. 1 | Microbial Cell Factories

Fig. 1

From: A novel cold-adapted esterase from Enterobacter cloacae: Characterization and improvement of its activity and thermostability via the site of Tyr193Cys

Fig. 1

a Multiple sequence alignments of Lip and the other two esterases (Este1 PDB: 2C7B_A, Este2 PDB: 1EVQ_A). The strictly conserved residues are filled with blue and the catalytic triads are indicated by the red triangles and the conserved motif is indicated by the red box. b SDS-PAGE analysis of Lip and mutants (10 µl; protein concentration 7 µg). M: Protein molecular weight marker; 1: The wild type Lip protein; 2: The mutant V29A/ Y193C protein; 3: The mutant V29A protein; 4: The mutant Y193C protein

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