Table 1 Yeast strains constructed in this study

YPH499

MATa URA3-52 LYS2-801 ADE2-101 TRP1-Δ63 HIS3-Δ200 LEU2-Δ1

BY4741

MATa HIS3Δ1 LEU2Δ0 MET15Δ0 URA3Δ0

YA0K0

YPH499/pGK426/pGK423

YA0K1

YPH499/pGK426/pGK423-kivd

YA0K2

YPH499/pGK426/pGK423-ARO10

YA0K3

YPH499/pGK426/pGK423-THI3

YA1K0

YPH499/pGK426-ADH1/pGK423

YA1K1

YPH499/pGK426-ADH1/pGK423-kivd

YA1K2

YPH499/pGK426-ADH1/pGK423-ARO10

YA1K3

YPH499/pGK426-ADH1/pGK423-THI3

YA2K0

YPH499/pGK426-ADH2/pGK423

YA2K1

YPH499/pGK426-ADH2/pGK423-kivd

YA2K2

YPH499/pGK426-ADH2/pGK423-ARO10

YA2K3

YPH499/pGK426-ADH2/pGK423-THI3

YA3K0

YPH499/pGK426-ADH5/pGK423

YA3K1

YPH499/pGK426-ADH5/pGK423-kivd

YA3K2

YPH499/pGK426-ADH5/pGK423-ARO10

YA3K3

YPH499/pGK426-ADH5/pGK423-THI3

YA4K0

YPH499/pGK426-ADH6/pGK423

YA4K1

YPH499/pGK426-ADH6/pGK423-kivd

YA4K2

YPH499/pGK426-ADH6/pGK423-ARO10

YA4K3

YPH499/pGK426-ADH6/pGK423-THI3

YA5K0

YPH499/pGK426-ADH7/pGK423

YA5K1

YPH499/pGK426-ADH7/pGK423-kivd

YA5K2

YPH499/pGK426-ADH7/pGK423-ARO10

YA5K3

YPH499/pGK426-ADH7/pGK423-THI3

YA6K0

YPH499/pGK426-SFA1/pGK423

YA6K1

YPH499/pGK426-SFA1/pGK423-kivd

YA6K2

YPH499/pGK426-SFA1/pGK423-ARO10

YA6K3

YPH499/pGK426-SFA1/pGK423-THI3

Y06C250

YPH499/pGK406-cimA/pATP425

Y06C25C

YPH499/pGK406-cimA/pATP425-leuC(Cb)-leuD(Cb)

Y06C25E

YPH499/pGK406-cimA/pATP425-leuC(Ec)-leuD(Ec)

Y06C25 M

YPH499/pGK406-cimA/pATP425-leuC(Mj)-leuD(Mj)

Y26C250

YPH499/pGK426-cimA/pATP425

Y26C25C

YPH499/pGK426-cimA/pATP425-leuC(Cb)-leuD(Cb)

Y26C25E

YPH499/pGK426-cimA/pATP425-leuC(Ec)-leuD(Ec)

Y26C25 M

YPH499/pGK426-cimA/pATP425-leuC(Mj)-leuD(Mj)

Y5040

YPH499/pATP425/pATP424

Y5041

YPH499/pATP425/pATP424-ILV1

Y5042

YPH499/pATP425/pATP424-tdcB

Y5043

YPH499/pATP425/pATP424-ilvA

Y5C40

YPH499/pATP425-cimA-leuC(Cb)-leuD(Cb)/pATP424

Y5C41

YPH499/pATP425-cimA-leuC(Cb)-leuD(Cb)/pATP424-ILV1

Y5C42

YPH499/pATP425-cimA-leuC(Cb)-leuD(Cb)/pATP424-tdcB

Y5C43

YPH499/pATP425-cimA-leuC(Cb)-leuD(Cb)/pATP424-ilvA

Y5E40

YPH499/pATP425-cimA-leuC(Ec)-leuD(Ec)/pATP424

Y5E41

YPH499/pATP425-cimA-leuC(Ec)-leuD(Ec)/pATP424-ILV1

Y5E42

YPH499/pATP425-cimA-leuC(Ec)-leuD(Ec)/pATP424-tdcB

Y5E43

YPH499/pATP425-cimA-leuC(Ec)-leuD(Ec)/pATP424-ilvA

Y5M40

YPH499/pATP425-cimA-leuC(Mj)-leuD(Mj)/pATP424

Y5M41

YPH499/pATP425-cimA-leuC(Mj)-leuD(Mj)/pATP424-ILV1

Y5M42

YPH499/pATP425-cimA-leuC(Mj)-leuD(Mj)/pATP424-tdcB

Y5M43

YPH499/pATP425-cimA-leuC(Mj)-leuD(Mj)/pATP424-ilvA

B50

BY4741/pATP425

B5C

BY4741/pATP425-cimA-leuC(Cb)-leuD(Cb)

B5E

BY4741/pATP425-cimA-leuC(Ec)-leuD(Ec)

B5M

BY4741/pATP425-cimA-leuC(Mj)-leuD(Mj)

BG5C

BY4741ΔGLY1/pATP425-cimA-leuC(Cb)-leuD(Cb)

BG5E

BY4741ΔGLY1/pATP425-cimA-leuC(Ec)-leuD(Ec)

BG5M

BY4741ΔGLY1/pATP425-cimA-leuC(Mj)-leuD(Mj)

YG5040

YPH499ΔGLY1/pATP425/pATP424

YG5C42

YPH499ΔGLY1/pATP425-cimA-leuC(Cb)-leuD(Cb)/pATP424-tdcB

YG5E42

YPH499ΔGLY1/pATP425-cimA-leuC(Ec)-leuD(Ec)/pATP424-tdcB

YG5M42

YPH499ΔGLY1/pATP425-cimA-leuC(Mj)-leuD(Mj)/pATP424-tdcB

YG504030

YPH499ΔGLY1/pATP425/pATP424/pATP423

YG5C4231

YPH499ΔGLY1/pATP425-cimA-leuC(Cb)-leuD(Cb)/pATP424-tdcB/pATP423-thrA-thrB-thrC

YG5C4232

YPH499ΔGLY1/pATP425-cimA-leuC(Cb)-leuD(Cb)/pATP424-tdcB-asd/pATP423-thrA-thrB-thrC