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Table 1 Yeast strains constructed in this study

From: Metabolic engineering of the 2-ketobutyrate biosynthetic pathway for 1-propanol production in Saccharomyces cerevisiae

Strains Genotypes
YPH499 MATa URA3-52 LYS2-801 ADE2-101 TRP1-Δ63 HIS3-Δ200 LEU2-Δ1
BY4741 MATa HIS3Δ1 LEU2Δ0 MET15Δ0 URA3Δ0
YA0K0 YPH499/pGK426/pGK423
YA0K1 YPH499/pGK426/pGK423-kivd
YA0K2 YPH499/pGK426/pGK423-ARO10
YA0K3 YPH499/pGK426/pGK423-THI3
YA1K0 YPH499/pGK426-ADH1/pGK423
YA1K1 YPH499/pGK426-ADH1/pGK423-kivd
YA1K2 YPH499/pGK426-ADH1/pGK423-ARO10
YA1K3 YPH499/pGK426-ADH1/pGK423-THI3
YA2K0 YPH499/pGK426-ADH2/pGK423
YA2K1 YPH499/pGK426-ADH2/pGK423-kivd
YA2K2 YPH499/pGK426-ADH2/pGK423-ARO10
YA2K3 YPH499/pGK426-ADH2/pGK423-THI3
YA3K0 YPH499/pGK426-ADH5/pGK423
YA3K1 YPH499/pGK426-ADH5/pGK423-kivd
YA3K2 YPH499/pGK426-ADH5/pGK423-ARO10
YA3K3 YPH499/pGK426-ADH5/pGK423-THI3
YA4K0 YPH499/pGK426-ADH6/pGK423
YA4K1 YPH499/pGK426-ADH6/pGK423-kivd
YA4K2 YPH499/pGK426-ADH6/pGK423-ARO10
YA4K3 YPH499/pGK426-ADH6/pGK423-THI3
YA5K0 YPH499/pGK426-ADH7/pGK423
YA5K1 YPH499/pGK426-ADH7/pGK423-kivd
YA5K2 YPH499/pGK426-ADH7/pGK423-ARO10
YA5K3 YPH499/pGK426-ADH7/pGK423-THI3
YA6K0 YPH499/pGK426-SFA1/pGK423
YA6K1 YPH499/pGK426-SFA1/pGK423-kivd
YA6K2 YPH499/pGK426-SFA1/pGK423-ARO10
YA6K3 YPH499/pGK426-SFA1/pGK423-THI3
Y06C250 YPH499/pGK406-cimA/pATP425
Y06C25C YPH499/pGK406-cimA/pATP425-leuC(Cb)-leuD(Cb)
Y06C25E YPH499/pGK406-cimA/pATP425-leuC(Ec)-leuD(Ec)
Y06C25 M YPH499/pGK406-cimA/pATP425-leuC(Mj)-leuD(Mj)
Y26C250 YPH499/pGK426-cimA/pATP425
Y26C25C YPH499/pGK426-cimA/pATP425-leuC(Cb)-leuD(Cb)
Y26C25E YPH499/pGK426-cimA/pATP425-leuC(Ec)-leuD(Ec)
Y26C25 M YPH499/pGK426-cimA/pATP425-leuC(Mj)-leuD(Mj)
Y5040 YPH499/pATP425/pATP424
Y5041 YPH499/pATP425/pATP424-ILV1
Y5042 YPH499/pATP425/pATP424-tdcB
Y5043 YPH499/pATP425/pATP424-ilvA
Y5C40 YPH499/pATP425-cimA-leuC(Cb)-leuD(Cb)/pATP424
Y5C41 YPH499/pATP425-cimA-leuC(Cb)-leuD(Cb)/pATP424-ILV1
Y5C42 YPH499/pATP425-cimA-leuC(Cb)-leuD(Cb)/pATP424-tdcB
Y5C43 YPH499/pATP425-cimA-leuC(Cb)-leuD(Cb)/pATP424-ilvA
Y5E40 YPH499/pATP425-cimA-leuC(Ec)-leuD(Ec)/pATP424
Y5E41 YPH499/pATP425-cimA-leuC(Ec)-leuD(Ec)/pATP424-ILV1
Y5E42 YPH499/pATP425-cimA-leuC(Ec)-leuD(Ec)/pATP424-tdcB
Y5E43 YPH499/pATP425-cimA-leuC(Ec)-leuD(Ec)/pATP424-ilvA
Y5M40 YPH499/pATP425-cimA-leuC(Mj)-leuD(Mj)/pATP424
Y5M41 YPH499/pATP425-cimA-leuC(Mj)-leuD(Mj)/pATP424-ILV1
Y5M42 YPH499/pATP425-cimA-leuC(Mj)-leuD(Mj)/pATP424-tdcB
Y5M43 YPH499/pATP425-cimA-leuC(Mj)-leuD(Mj)/pATP424-ilvA
B50 BY4741/pATP425
B5C BY4741/pATP425-cimA-leuC(Cb)-leuD(Cb)
B5E BY4741/pATP425-cimA-leuC(Ec)-leuD(Ec)
B5M BY4741/pATP425-cimA-leuC(Mj)-leuD(Mj)
BG5C BY4741ΔGLY1/pATP425-cimA-leuC(Cb)-leuD(Cb)
BG5E BY4741ΔGLY1/pATP425-cimA-leuC(Ec)-leuD(Ec)
BG5M BY4741ΔGLY1/pATP425-cimA-leuC(Mj)-leuD(Mj)
YG5040 YPH499ΔGLY1/pATP425/pATP424
YG5C42 YPH499ΔGLY1/pATP425-cimA-leuC(Cb)-leuD(Cb)/pATP424-tdcB
YG5E42 YPH499ΔGLY1/pATP425-cimA-leuC(Ec)-leuD(Ec)/pATP424-tdcB
YG5M42 YPH499ΔGLY1/pATP425-cimA-leuC(Mj)-leuD(Mj)/pATP424-tdcB
YG504030 YPH499ΔGLY1/pATP425/pATP424/pATP423
YG5C4231 YPH499ΔGLY1/pATP425-cimA-leuC(Cb)-leuD(Cb)/pATP424-tdcB/pATP423-thrA-thrB-thrC
YG5C4232 YPH499ΔGLY1/pATP425-cimA-leuC(Cb)-leuD(Cb)/pATP424-tdcB-asd/pATP423-thrA-thrB-thrC