Translation efficiency of heterologous proteins is significantly affected by the genetic context of RBS sequences in engineered cyanobacterium Synechocystis sp. PCC 6803

Table 1 RBSs subjected to in vivo characterization or comparative analysis in Synechocystis sp. PCC 6803 as reported in literature

Name and sequence	Organism/source	Reporter	References
BBa_B0029 ATTCACACAGGAAACCTACTAGATG NTTCACACAGGAAACCTCTAGAATG	BioBrick	mTagBFP EYFP	[6] [6]
BBa_B0030 (N)_nATTAAAGAGGAGAAA(N)_nATG AATTAAAGAGGAGAAATACTAGATG NATTAAAGAGGAGAAATCTAGAATG	BioBrick	GFPmut3b mTagBFP EYFP	[4] [6] [6]
BBa_B0031 GATCACACAGGAAACCTACTAGATG NNTCACACAGGAAACCTCTAGAATG	BioBrick	mTagBFP EYFP	[6] [6]
BBa_B0032 (N)_nTCACACAGGAAAG(N)_nATG AGATCACACAGGAAAGTACTAGATG NNNTCACACAGGAAAGTCTAGAATG	BioBrick	GFPmut3b mTagBFP EYFP	[4] [6] [6]
BBa_B0033 CTAGATCACACAGGACTACTAGATG NNNNNTCACACAGGACTCTAGAATG	BioBrick	mTagBFP EYFP	[6] [6]
BBa_B0034 (N)_n AAAGAGGAGAAA(N)_nATG TAGAAAAGAGGAGAAATACTAGATG NNNNAAAGAGGAGAAATCTAGAATG	BioBrick	GFPmut3b mTagBFP EYFP	[4] [6] [6]
BBa_B0035 GAATTAAAGAGGAGAATACTAGATG NNATTAAAGAGGAGAATCTAGAATG	BioBrick	mTagBFP EYFP	[6] [6]
BBa_B0064 TAGAAAAGAGGGGAAATACTAGATG NNNNAAAGAGGGGAAATCTAGAATG	BioBrick	mTagBFP EYFP	[6] [6]
psbA2 RBS AGACATAAGGAATTATTACTAGATG NNNCATAAGGAATTATTCTAGAATG	psbA2 ^a	mTagBFP EYFP	[6] [6]
rbcL RBS AGATTATGGAGGACTGTACTAGATG NNNTTATGGAGGACTGTCTAGAATG	rbcL ^a	mTagBFP EYFP	[6] [6]
RBS* NNNNNNTAGTGGAGGTNNNNNNATG TCTAGATAGTGGAGGTTACTAGATG NNNNNNTAGTGGAGGTTCTAGAATG	A synthetic RBS^a	GFPmut3b mTagBFP EYFP	[4] [6] [6]
Unnamed NA/GAAGGAGGTNNNNNNNNNNNNATG	A synthetic RBS	eCFP and YFP	[5]
RBSv4 AAAATAAAGAAGGAGGAACAGCATG	A synthetic RBS	Synthetic efe	[7]

The sequences (22 bp upstream from the initiation codon) are presented in 5′–3′ direction, with the core SD consensus sequence GGAGG underlined and ATG shown in bold. The nucleotides that have not been specified in the corresponding publications are denoted as N. Comparative studies in which the RBS is not clearly distinguished from the context of the promoter have not been included
^aIndicates that the corresponding gene has been used also as the source of RBS sequence in this study

ISSN: 1475-2859