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Fig. 3 | Microbial Cell Factories

Fig. 3

From: Translation efficiency of heterologous proteins is significantly affected by the genetic context of RBS sequences in engineered cyanobacterium Synechocystis sp. PCC 6803

Fig. 3

Evaluation of the translational efficiencies of selected RBS sequences based on a ethylene productivity of six designed strains expressing ethylene-forming enzyme measured from the headspace at 4 h after induction. The bars showing the average ethylene productivity (nL C2H2/ml culture/h) and standard deviations were calculated from three biological replicates (n = 3) and normalized to OD750 nm. The arrows indicate the RBSs which differ the most in comparison to the fluorescent datasets in Fig. 1. b Salis calculator prediction for the translation initiation rate using the nucleotide sequence around the start codon (− 25 to + 35) as input, displaying predicted translation initiation rates for all the RBSs in combination with the two fluorescent reporters sYFP2 (yellow) and GFPmut3b (green)

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Microbial Cell Factories

ISSN: 1475-2859

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