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Fig. 1 | Microbial Cell Factories

Fig. 1

From: Beyond antibody engineering: directed evolution of alternative binding scaffolds and enzymes using yeast surface display

Fig. 1

Yeast surface display setup as pioneered by Boder and Wittrup in 1997 [1]. The protein of interest (a vNAR domain in this particular depiction) is fused to the C-terminus of the Aga2p protein. Aga2p is covalently linked to Aga1p via two disulfide bonds. Aga1p anchors the fusion protein to the cell wall, ensuring a genotype–phenotype coupling of individual yeast cells. The vNAR structure has been modified from pdb identifier 4GHK using UCSF Chimera [133]

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