Fig. 1
From: Directly mining a fungal thermostable α-amylase from Chinese Nong-flavor liquor starter
Schematic representation, genomic integration, Zymogram analyses and plate-based activity assays of NFAmy13A. a Schematic representation of NFAmy13A. b Verification of integration of NFAmy13A gene into genome of P. pastoris X33. c Zymogram analyse of amylase activities of native NFAmy13A. d Plate-based activity assay of native NFAmy13A. The signal peptide is represented by the filled rectangle. GH13: family 13 glycoside hydrolase domain. GUF1966: domain of unknown function 1966. Lane M, molecular mass markers; Lane 1, purified native NFAmy13A; Lane 2, zymogram of purified native NFAmy13A