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Fig. 2 | Microbial Cell Factories

Fig. 2

From: Comparative immunogenicity of preparations of yeast-derived dengue oral vaccine candidate

Fig. 2

Schematic representation of the oral immunization protocol. Each mouse in a group of six was orally gavaged with either 1.6 g fresh weight of cells resuspended in a final volume of 2.4 mL of PBS or an equivalent amount of TSP in CFE preparations of recombinant yeast cells. For each dose, the WC or CFE suspensions were split equally and administered four times per day. Oral immunization was administered every alternate week up to 4 weeks. Subsequent immune response monitoring was performed through retro-orbital bleeding and fecal matter collection at 4 days after the final feeding and continued every week up to 32 days, followed by intraperitoneal booster injection with purified antigen on day 43 and subsequent bleeding on day 46. ‘d#’ represents days post final vaccination

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