Table 3 Oligonucleotide primers used in this study
Primer | Sequence (5′–3′)a | Restriction site |
|---|---|---|
H1F | GAAGAAGATCATTTACTCGAG | XhoI |
H1R | TACCGTTCGTATAGCATACATTATACGAAGTTATCAGAACGCAAATCTCTTCTA | Â |
H2F | TGTATGCTATACGAACGGTAGTCGACTTACTTAATGCTATTCATTA | SalI |
H2R | TTAAGATCTACATCGAGTTCAGCAAGCTAA | BglII |
H3F | ATATGTCGACATTACAGTTACAAGCCATATAC | SalI |
H3R | TCAATATTCTTCTCCGTCCC | Â |
H4F | GGGACGGAGAAGAATATTGAAGATCTCACTGCAGTATATCAGGTAACAAAAAGT | BglII, SalI |
H4R | ACATGCATGCTTCCCTCTCCAAATATGCAC | SphI |
H5F | CGGGATCCAATAGCTCAGATTTTTAACAACA | BamHI |
H5R | TACCGTTCGTATAGCATACATTATACGAAGTTATTAATTTATCAAAAACCTTATTC | Â |
H6R | CCCAAGCTTGTACATCATACTGTTCATGCC | HindIII |
H6F | TGTATGCTATACGAACGGTACTCGAGAAGCCATCGCTGCTAACAAA | XhoI |
H7F | TTTTGGCCAAGCTGGTTTT | Â |
H7R | TTGTTTTTTCGCGGTACTGA | Â |
H8F | TCAGTACCGCGAAAAAACAAGATCTCACACTCGAGTGTAGTCGATAAAAATT | BglII, XhoI |
H8R | GCA ACATGCATGCAGACCATGAGACTATTTCAG | SphI |
PrF | TATAGCATACATTATACGAAGTTATCCAGCTAGGCCTAGTGTCCGT | Â |
PrR | CGTCAATACCTCCTAATTGA | Â |
galEF | TCAATTAGGAGGTATTGACGATGACAATTGCAGTTTTAGG | Â |
galER | TTACTCGAGTCAATTCCGGTCACCAAATC | XhoI |
catF1 | CCGCTCGAGATAACTTCGTATAATGTATGCTATA | XhoI |
catR1 | CCGCTCGAGATAACTTCGTATAGCATACATTATA | XhoI |
catF2 | CCGCTCGAGATAACTTCGTATAGCATACATTATACGAACGGTACGAAAGTCGACGGCAATAG | XhoI |
catR2 | GGAAGATCTCTGTAATATAAAAACCTTCTTC | BglII |