Table 2 Plasmids and bacterial strains used in this study

Strains

 Escherichia coli DH5α

F⁻ supE44 ∆lacU169 Ф80lacZ ∆M15 hsdR17 recA1 endA1 gyrA96 thi-1 relA1

Novagen

 Lactobacillus casei BL23

Derivative of L. casei ATCC 393 (pLZ15⁻)

[44]

Plasmids

 pG⁺host9

Erm^r; temperature-sensitive vector

[17]

 pUC19

Amp^r; cloning vector

This study

 pSec:Leiss:Nuc

pWV01 replicon; expresses Nuc under P_nisA control; Cm^r

[28]

 pET-28a

Kan^r; cloning vector

This study

 pTRKH2

Erm^r; theta-replicating vector

[29]

 pMSP456

Expression LCABL_13040-50-60 under P_nisA control

This study

 pMSPcre

Expression Cre under P_nisA control

This study

 pUCgalK

Source of fragment loxP-cat-loxP

[15]

 pCD4033-gfp

Gene gfp as a reporter in the downstream of P_ldh

[45]