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Fig. 4 | Microbial Cell Factories

Fig. 4

From: Coupling the recombineering to Cre-lox system enables simplified large-scale genome deletion in Lactobacillus casei

Fig. 4

Sequential deletion of double large-scale genome regions in L. casei BL23. a Genomic deletions in the double-deletion mutants of L. casei BL23. The red boxes represent the 1.68% deleted regions. b Colony PCR results. M: DNA marker; C1: L. casei BL23 (12.8 kb); C2: L. casei BL23 (39.3 kb); 1: random picked chloramphenicol resistance colonies (12.8 kb); 2: random picked chloramphenicol resistance colonies (39.3 kb)

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Microbial Cell Factories

ISSN: 1475-2859

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